Calibration Method of Fluorescence Excited Light Path in Nucleic Acid Detection System

Manshu Du; Xiaohui Lin; Jiayu Yang; Jianchun Liu; Dongxu Zhang

doi:10.3788/LOP202259.0712003

Laser & Optoelectronics Progress, Volume. 59, Issue 7, 0712003(2022)

Calibration Method of Fluorescence Excited Light Path in Nucleic Acid Detection System

Manshu Du^1,2, Xiaohui Lin^1、*, Jiayu Yang², Jianchun Liu¹, and Dongxu Zhang²

Author Affiliations

¹School of Electrical Engineering and Automation, Xiamen Institute of Technology, Xiamen , Fujian 361024, China

²Shool of Public Health, Xiamen University, Xiamen , Fujian 361102, China

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Figures & Tables(11)

Fig. 1. Structure diagram of optical system of nucleic acid detection instrument

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Fig. 2. Schematic of excitation light path

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$Relation curve between exit angle of light source and radius of light at edge of secondary refraction surface$

Fig. 3. Relation curve between exit angle of light source and radius of light at edge of secondary refraction surface

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$Relation curve between exit angle of light source and exit angle of secondary refraction surface$

Fig. 4. Relation curve between exit angle of light source and exit angle of secondary refraction surface

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Fig. 5. ZEMAX simulation results

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Fig. 6. Experimental setup for measuring light spot

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Fig. 7. Images taken by CCD with five different apertures

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Fig. 8. Changes of exit spot radius of excitation light path under different apertures

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Fig. 9. Images taken by CCD of optical path collimation experiments under different distances between screen and lens. (a) 7.7 mm; (b) 8.7 mm

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Table 1. Parameters table of numerical calculation system for spot radius
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Table 1. Parameters table of numerical calculation system for spot radius
r₁ /mm r₂ /mm n₁ n₂ l/mm g/mm
5.17 ∞ 1.0 1.5 7.3 7.7

Table 2. Experimental data of collimation of excitation light path

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Table 2. Experimental data of collimation of excitation light path

Experiment	Distance /mm	Spot radius /dpi	Reference size /dpi	Actual size /mm	Exit angle /（°）
1	7.7	24.97	11.4	2.19	-2.86
1	8.7	24.38	11.4	2.14	-2.86
2	7.7	24.69	11.2	2.20	-1.72
2	8.7	24.79	11.4	2.17	-1.72
3	7.7	24.79	11.3	2.19	-0.57
3	8.7	24.66	11.3	2.18	-0.57

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Manshu Du, Xiaohui Lin, Jiayu Yang, Jianchun Liu, Dongxu Zhang. Calibration Method of Fluorescence Excited Light Path in Nucleic Acid Detection System[J]. Laser & Optoelectronics Progress, 2022, 59(7): 0712003

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