An Analytical Model for Multi-Wavelength Transmittance Spectra of Bacteria in Water

Yuxia Hu; Nanjing Zhao; Tingting Gan; Jingbo Duan; Huijuan Yu; Deshuo Meng; Jianguo Liu; Wenqing Liu

doi:10.3788/AOS201737.0830001

Acta Optica Sinica, Volume. 37, Issue 8, 0830001(2017)

An Analytical Model for Multi-Wavelength Transmittance Spectra of Bacteria in Water

Yuxia Hu^1,2,3, Nanjing Zhao^1,3、*, Tingting Gan^1,3, Jingbo Duan^1,3, Huijuan Yu^1,2,3, Deshuo Meng^1,3, Jianguo Liu^1,3, and Wenqing Liu^1,3

Author Affiliations

¹ Key Laboratory of Environment Optics and Technology, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei, Auhui 230031, China

² University of Science and Technology of China, Hefei, Auhui 230026, China

³ Anhui Key Laboratory of Environment Optical Detection Technology, Hefei, Anhui 230031, China

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Figures & Tables(8)

Fig. 1. Multi-wavelength transmission spectrum of Escherichia coli

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Fig. 2. Absorption coefficients of total nucleic acid, chromophoric amino acid, and dipicolinic acid in bacterial microorganism[13]

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$Imaginary part of complex refractive index of (a) chromophoric amino acid and (b) total nucleic acid$

Fig. 3. Imaginary part of complex refractive index of (a) chromophoric amino acid and (b) total nucleic acid

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$Variation in real part of complex refractive index of (a) chromophoric amino acid and (b) total nucleic acid$

Fig. 4. Variation in real part of complex refractive index of (a) chromophoric amino acid and (b) total nucleic acid

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Fig. 5. Comparision between measured and calculated optical density spectra of Escherichia coli

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Fig. 6. Correlation between calculated spectra and measured spectra of single Escherichia coli cell

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Table 1. Relative error between measured and calculated optical density spectra in different bands
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Table 1. Relative error between measured and calculated optical density spectra in different bands
Wavelength /nm 240-250 250-400 400-750 750-900 240-900
Maximum relative error /% 6.18 3.11 3.12 9.76 9.76
Average relative error /% 2.09 0.99 0.97 3.67 1.62

Table 2. Calculated parameters from the transmittance model in 250-750 nm band for Escherichia coli

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Table 2. Calculated parameters from the transmittance model in 250-750 nm band for Escherichia coli

Parameter	Calculated value	Literature/experimental value
Volume /μm³	4.6093	1-7^[23]
Average diameter of external structure $\begin{matrix} {D_{1}}_{} \end{matrix}$ /μm	2.0648	1.9189
Particle number $\begin{matrix} {N_{p}}_{} \end{matrix}$ /mL^-1	20497000	20000000
Average diameter of internal structure $\begin{matrix} {D_{2}}_{} \end{matrix}$ /μm	0.0667
Volume fraction of internal structure /%	30

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Yuxia Hu, Nanjing Zhao, Tingting Gan, Jingbo Duan, Huijuan Yu, Deshuo Meng, Jianguo Liu, Wenqing Liu. An Analytical Model for Multi-Wavelength Transmittance Spectra of Bacteria in Water[J]. Acta Optica Sinica, 2017, 37(8): 0830001

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Paper Information

Category: Spectroscopy

Received: Jan. 10, 2017

Accepted: --

Published Online: Sep. 7, 2018

The Author Email: Zhao Nanjing (njzhao@aiofm.ac.cn)

DOI:10.3788/AOS201737.0830001

Topics

laser devices and laser physics

Lasers and Laser Optics

Laser physics

laser manufacturing

Instrumentation, Measurement and Metrology

Table 1. Relative error between measured and calculated optical density spectra in different bands

Table 1. Relative error between measured and calculated optical density spectra in different bands

Table 2. Calculated parameters from the transmittance model in 250-750 nm band for Escherichia coli

Table 2. Calculated parameters from the transmittance model in 250-750 nm band for Escherichia coli