Chinese Optics, Volume. 17, Issue 1, 150(2024)

NIR-II fluorescence confocal imaging based on indirect wavefront shaping

Tian TAN1,2, Tian-yue SHI2, Chang-feng WU2, and Hong-shang PENG1、*
Author Affiliations
  • 1Engineering Research Center of Photonic Design Software Ministry of Education, College of Science, Minzu University of China, Beijing 100081, China
  • 2Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen 518055, China
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    Figures & Tables(7)
    Absorption and emission spectra of the semiconductor polymer fluorescent probes
    Near-infrared laser scanning confocal microscope based on indirect wavefront sensing
    Feedback system for controlling SLM
    Testing and correction of the system aberrations. (a) Imaging of samples without correction; (b) imaging of samples after systematic aberration correction; (c) curve of the evaluation function as a function of iterative order; (d) the corrected phase diagram calculated by GA;scale: 200 μm in the full field of view and 20 μm in the local magnification of the region of interest in (a) (b)
    Aberration correction results of electrospinning. (a) Image with a 30 μm air plate and performing only system aberration correction; (b) in the case of imaging in figure (a), aberration correction caculated by using DASH; (c) in the case of imaging in figure (a), aberration correction caculated by using GA; (d) fluorescence intensity distribution at the white line marker in partial enlarged pictures of the region of interest in figures (a) (b) (c); (e) the corrected phase map calculated by DASH; (f) the corrected phase diagram calculated by GA;Scale: 200 μm in the full field of view and 20 μm in the local magnification of the region of interest in (a) (b) (c)
    Aberration correction results of scattering medium. (a) Direct imaging; (b) image after adding scattering medium; (c) image after system aberration correction; (d) image after total aberration correction; (e) distribution of fluorescence intensity at white line markers in partial enlarged pictures of (a)-(d); (f) the corrected phase map calculated by GA; Scale: 200 μm in the full field of view and 20 μm in the local magnification of the region of interest in (a) (b) (c) (d)
    Intracranial imaging results of living mice. (a) Image of intracranial blood vessels in mice with only systematic aberration correction; (b) image of intracranial blood vessels in mice with full aberration correction; (c) the fluorescence intensity distribution at the white line markers in (a) and (b); (d) curve of the evaluation function as a function of iterative order; scale: 200 μm in (a)(b)
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    Tian TAN, Tian-yue SHI, Chang-feng WU, Hong-shang PENG. NIR-II fluorescence confocal imaging based on indirect wavefront shaping[J]. Chinese Optics, 2024, 17(1): 150

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    Paper Information

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    Received: Apr. 18, 2023

    Accepted: --

    Published Online: Mar. 28, 2024

    The Author Email:

    DOI:10.37188/CO.2023-0070

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