Fig. 1. Schematic diagram of 3D light-sheet microscopy imaging system based on liquid zoom lens and galvanometer mirror

Fig. 2. Object-image relationship of double telecentric optical path

Fig. 3. Control principal diagram of optical slice microscopic system

Fig. 4. Images formed by periodic fringe mask and magnification of microscopic imaging system during zooming process of liquid lens. (a) I_ETL=0 mA; (b) I_ETL=60 mA; (c) I_ETL=120 mA; (d) I_ETL=180 mA; (e) I_ETL=240 mA; (f) I_ETL=293 mA; (g) magnification is 9.09

Fig. 5. Relationship between distance of main plane of object and applied current I_ETL of liquid zoom lens

Fig. 6. Gaussian beam, Gaussian light sheets, and relationship between position of scanning light sheet of scanning galvanometer and applied voltage V_{G_M}. (a) Gaussian beam; (b) Gaussian light sheet; Gaussian light sheets detected by optical beam profilers for (c) V_{G_M}=0 mV, (d) V_{G_M}=50 mV, (e) V_{G_M}=100 mV, (f) V_{G_M}=150 mV, (g) V_{G_M}=200 mV, (h) V_{G_M}=250 mV, (i) V_{G_M}=300 mV, (j) V_{G_M}=350 mV, and (k) V_{G_M}=400 mV; (l) relationship between position of scanning light sheet of scanning galvanometer and applied voltage

Fig. 7. Synchronous scanning relationship between galvanometer mirror and liquid zoom lens. (a) Flow chart for measuring synchronous scanning relationship between galvanometer mirror and liquid zoom lens; (b) corresponding relationship between applied voltage of galvanometer mirror V_{G_M} and current of liquid zoom lens, and corresponding position of main plane of object

Fig. 8. Sequence chart of control signal of synchronous control system

Fig. 9. Imaging results of fluorescent microsphere with diameter of 10 μm. (a) Two-dimensional image distribution of sample; (b) local magnified image of fluorescent microsphere in box in Fig. 9(a) and normalized intensity distribution curve and Gaussian fitting result

Fig. 10. Measurement results of system point spread function. (a) Three-dimensional reconstructed images of fluorescent microsphere sample with diameter of 0.5 μm projected along xy, yz, and xz planes; normalized intensity distributions of point spread function along (b) lateral and (c) axial directions and Gaussian fitting results

Fig. 11. Fluorescent microsphere images at different depth and 3D reconstruction image of fluorescent microsphere sample. (a) z=60 μm; (b) z=150 μm; (c) z=240 μm; (d) z=330 μm; (e) 3D reconstruction of fluorescent microsphere samples by volume rendering algorithm

Fig. 12. Imaging results of zebrafish embryo head at different depth

Fig. 13. Imaging results of zebrafish heart beating process