Chinese Journal of Lasers, Volume. 48, Issue 16, 1607002(2021)

Common-Path Parallel Fluorescence Emission Difference Super-Resolution Microscopy

Zhimin Zhang1, Yuran Huang1, Shaocong Liu1, Cuifang Kuang1,2,3、*, Liangcai Cao4, Yong Liu5, Yubing Han1, Xiang Hao1, and Xu Liu1
Author Affiliations
  • 1State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, China
  • 2Ningbo Research Institute, Zhejiang University, Ningbo, Zhejiang 315100, China
  • 3Collaborative Innovation Center of Extreme Optics, Shanxi University, Taiyuan, Shanxi 0 30006, China
  • 4State Key Laboratory of Precision Measurement Technology and Instrument, Department of Precision Instruments, Tsinghua University, Beijing 100084
  • 5Shanghai University of Electric Power, Shanghai 200090
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    Figures & Tables(4)
    System schematic. (a) System setup strategy for cpFED, where the SLM is conjugated to the entrance pupil of the objective lens and the sample surface is conjugated to the pinhole plane; (b) phase grayscale images of SLM
    Imaging of particle with diameter of 200 nm. (a) Solid spot (confocal) image; (b) hollow spot image, where the parts within the dotted box correspond to the same field of view; (c) cpFED image, and its size is the same as the part within the dotted boxes in (a) and (b); (d)--(f) enlarged images in the solid boxes of (a)--(c). The scale bars represent 1 μm
    Imaging of particle with diameter of 100 nm. (a) Confocal image; (b) cpFED image; (c) normalized intensity distributions along the section lines in (a1) and (b1); (a1) (a2) enlarged images of the white frames 1 and 2 depicted in (a); (b1) (b2) enlarged images of the white frames 1 and 2 depicted in (b). Adjacent particles that cannot be resolved in the confocal image can be resolved in the cpFED image, and the full width at half maximum reaches 133 nm. The scale bars represent 1 μm
    Vimentin imaging. (a) Confocal image; (b) cpFED image; (a1) (a2) enlarged images of the white frames 1 and 2 depicted in (a); (b1) (b2) enlarged images of the white frames 1 and 2 depicted in (b). The scale bars represent 1 μm
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    Zhimin Zhang, Yuran Huang, Shaocong Liu, Cuifang Kuang, Liangcai Cao, Yong Liu, Yubing Han, Xiang Hao, Xu Liu. Common-Path Parallel Fluorescence Emission Difference Super-Resolution Microscopy[J]. Chinese Journal of Lasers, 2021, 48(16): 1607002

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    Paper Information

    Category: biomedical photonics and laser medicine

    Received: Jan. 13, 2021

    Accepted: Mar. 1, 2021

    Published Online: Jul. 30, 2021

    The Author Email: Cuifang Kuang (cfkuang@zju.edu.cn)

    DOI:10.3788/CJL202148.1607002

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