Advanced Imaging, Volume. 2, Issue 5, 055001(2025)
3D Gaussian adaptive reconstruction for Fourier light-field microscopy On the Cover
Fig. 1. Principle of 3D Gaussian adaptive tomography (3DGAT). (a) The optical setup and forward imaging process of the FLFM system. (b) The schematic of the training pipeline of 3DGAT. NOP, native object plane; OBJ, objective lens; TL, tube lens; NIP, native image plane; FL, Fourier lens; MLA, microlens array; CAM, camera.
Fig. 2. Performance and resolution evaluation of 3DGAT on synthetic data. (a) Maximum intensity projections (MIPs) of synthetic fluorescent beads restored by RL deconvolution and 3DGAT, compared to the ground truth. (b), (c) Intensity profile comparisons of RL deconvolution (gray) and 3DGAT (red) with the ground truth (black) along the yellow dashed lines in (a). (d) MIPs of synthetic reticular structures reconstructed by RL deconvolution and 3DGAT, alongside the ground truth. (e) Intensity profile comparisons of RL deconvolution (gray) and 3DGAT (red) with the ground truth (black) along the yellow dashed line in (d). (f) Quantitative evaluation using PSNR, SSIM, and LPIPS metrics for RL deconvolution and 3DGAT across the depth range. (g), (h)
Fig. 3. Comparison between RL deconvolution and 3DGAT with different loss functions. (a) MIPs of the simulated dandelion sample restored by RL deconvolution and 3DGAT with different losses, such as MSE, MAE + SSIM, and MSE + FDL, compared with the ground truth. (b), (c) Enlarged views of the regions outlined by the dashed boxes in corresponding colors in (a). (d) Normalized intensity profiles of the ground truth (gray), RL deconvolution (brown), and 3DGAT with MSE loss (purple), MAE + SSIM loss (pink), and MSE + FDL loss (red) along the white dashed line in (b). (e) Quantitative metrics for reconstruction evaluation across the depth range. Scale bar: (a) 100 µm; (b), (c) 20 µm.
Fig. 4. Reconstruction of experimentally captured zebrafish data. (a) Raw Fourier light-field image of zebrafish data acquired from Ref. [28]. (b)
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Chenyu Xu, Zhouyu Jin, Chengkang Shen, Hao Zhu, Zhan Ma, Bo Xiong, You Zhou, Xun Cao, Ning Gu, "3D Gaussian adaptive reconstruction for Fourier light-field microscopy," Adv. Imaging 2, 055001 (2025)
Category: Letter
Received: Apr. 1, 2025
Accepted: Aug. 21, 2025
Published Online: Feb. 28, 2025
The Author Email: Bo Xiong (xiongbo@pku.edu.cn), You Zhou (zhouyou@nju.edu.cn)