Fig. 1. Two-photon nonlinear structured illumination microscope system. HWP, half-wave plate; EOM, electro-optic modulator; L1, f = 60 mm; L2, f = 50 mm; GS, galvo scanner; DM, dichroic mirror; PMT, photo multiplier tube.

Fig. 2. Imaging of COS7 cell mitochondria. Scale bar: 2 µm. (A) Two-photon image, (B) super-resolution reconstructed image, and (C) intensity profile along the same white dashed line in the white dashed boxes in (A) and (B). The FWHM values of the two-photon and super-resolution reconstructed images are 260 and 82 nm, respectively.

Fig. 3. Imaging of COS7 cell mitochondria. Scale bar: 2 µm. (A) Two-photon image, (B) super-resolution reconstructed image, and (C) dynamic images within the white dashed boxes in (A) and (B).

Fig. 4. Image and analysis of mitochondria in H9C2 cells. (A) Mitochondrial imaging and super-resolution imaging of H9C2 cells before and after induced aging. Scale bar: 2 µm. (B) Mitochondria analyzer analyzes mitochondrial images of H9C2 cells before and after super-resolution. (C) Mitochondria analyzer analyzes mitochondrial images of H9C2 cells before and after induced aging.

Fig. 5. Mitochondrial dynamic imaging and super-resolution dynamic imaging of H9C2 cells after induced aging. (A) Two-photon image. (B) Super-resolution image. Scale bar: 2 µm. (C) Dynamic images with green dashed boxes in (A) and (B), with intervals of 5 s between each image. (D) Dynamic images with white dashed boxes in (A) and (B), with intervals of 5 s between each image.