Fig. 1. Light path of daul-optical path observing system for tracking free-moving C. elegans

Fig. 2. Structure of daul-optical path observing system for tracking free-moving C. elegans

Fig. 3. Images of USAF1951 resolution plates with different microscope light paths

Fig. 4. MTF curves of different combinations

Fig. 5. Fluorescent microsphere fluorescence image captured with 10× objective and enlarged image

Fig. 6. Bright field microscope images of N2 C. elegans

Fig. 7. Magnified images of C. elegans body with 40×objective

Fig. 8. Fluorescence microscope images of dhs-3p：：dhs-3：：GFP C. elegans

Fig. 9. Fluorescence images of different strains of C. elegans

Fig. 10. Petri dish image and morphological processing result（the strain of C. elegans is N2）