Fig. 1. Schematic of the HER2 test of FISH and structured-light illumination microscope (SIM) imaging system. (a) HER2 positive diagram; (b) HER2 negative diagram; (c) schematic of laser interferometric SIM imaging system

Fig. 2. SIM imaging on pathological tissue sections of breast cancer and quantitative analysis of imaging results. (a) SIM imaging result; (b) wide-field (WF) image corresponding to areas of dense signal distribution; (c) normalized intensity distribution at the line marker in Fig.2(b); (d) SIM image corresponding to areas of dense signal distribution; (e) normalized intensity distribution at the line marker in Fig.2(d)

Fig. 3. Stitched image obtained from SIM scanning. (a) Super-resolution large-field-of-view scanning stitched image; (b) multiple nuclei with low levels of HER2 gene amplification; (c) multiple nuclei with high levels of HER2 gene amplification

Fig. 4. Quantitative statistics of HER2 gene copy number in 20 typical cell nuclei