In this paper, the titanium (Ti)/hydroxyapatite (HA) powders collection experiment was designed using the unseparated powders width value as the component evaluation standard to overcome the problem of deviation between the actual composition and the designed composition after the two powders are mixed and sprayed in the laser cladding process. Based on the response surface method, the mathematical model between the width of the unseparated powders and process parameters of the laser was established. Through the calculation of the extreme value of the regression model and determination of the reasonable process parameters, the powders composition deviation in the laser deposition process was inhibited. The experimental results reveal that defocusing distance has the most significant influence on the Ti/HA powder separation, followed by the scanning speed and powder feeding rate. The unseparated powders width initially increases and then decreases with the increase of defocusing distance; meanwhile, it decreases with the increase of the scanning speed. Moreover, when the defocusing distance is -3 mm to -1.5 mm and the scanning speed is 0 to 4 mm·s -1, the width value of the unseparated powders is large, and the process parameters in this range help to improve the inaccurate composition raio of Ti/HA biomedical materilas.