Multispectral Microimaging System with Narrowband LED Illumination

Wang Cheng; Liu Bin; Zhou Chu; Li Nianning; Zhang Haonan; Xiang Huazhong; Zheng Gang; Wang Xiuli; Zhang Dawei

doi:10.3788/CJL202047.1207006

Chinese Journal of Lasers, Volume. 47, Issue 12, 1207006(2020)

Multispectral Microimaging System with Narrowband LED Illumination

Wang Cheng¹, Liu Bin¹, Zhou Chu², Li Nianning¹, Zhang Haonan², Xiang Huazhong¹, Zheng Gang¹, Wang Xiuli², and Zhang Dawei³

Author Affiliations

¹Key Laboratory of Medical Optical Technology and Instruments, Ministry of Education, Institute of Biomedical Optics and Optometry, University of Shanghai for Science and Technology, Shanghai 200093, China

²Institute of Photomedicine, Shanghai Skin Disease Hospital, Tongji University School of Medicine, Shanghai 200443, China

³Engineering Research Center of Optical Instrument and System, Ministry of Education, Key Laboratory of Modern Optical Systems, University of Shanghai for Science and Technology, Shanghai 200093, China

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Figures & Tables(12)

Fig. 1. Optical schematic diagram of the system

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Fig. 2. Picture of the experimental prototype

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Fig. 3. LED emission spectrum

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Fig. 4. Coupling design

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Fig. 5. Microscope graticules

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Fig. 6. USAF1951 resolution test target (group 7--6, 228lp/mm)

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Fig. 7. Single-band images of cancer tissue. (a) 480nm; (b) 520nm; (c) 600nm; (d) 660nm

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Fig. 8. Single-band images of normal tissue. (a) 480nm; (b) 520nm; (c) 600nm; (d) 660nm

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Fig. 9. False color image and spectra of cancer tissue

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Fig. 10. False color image and spectra of normal tissue

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Table 1. Coupling design parameters

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Table 1. Coupling design parameters

Surface		Curvature /mm	Thickness /mm	Glass	Semi-diameter /mm
OBJ	Standard	infinity	160	—	—
1	Standard	32.33	3	SK11	12
2	Standard	-105	2	SF19	12
3	Standard	infinity	129	—	12
4	Standard	30	5	—	8
5	Standard	-80	3	SK11	8
6	Standard	-90	97.47	SF19	8
IMA	Standard	infinity	—	—	5

Table 2. Sample spectral difference
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Table 2. Sample spectral difference
Group 1 2 3 4 5 6 7 8 9 10 Average
d_nn 0.31 0.35 0.39 0.38 0.43 0.33 0.35 0.32 0.27 0.53 0.37
d_cc 0.22 0.38 0.25 0.36 0.4 0.35 0.28 0.42 0.31 0.35 0.33
d_nc 1.24 1.02 1.31 0.96 0.99 0.94 1.47 1.19 1.15 1.11 1.14

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Wang Cheng, Liu Bin, Zhou Chu, Li Nianning, Zhang Haonan, Xiang Huazhong, Zheng Gang, Wang Xiuli, Zhang Dawei. Multispectral Microimaging System with Narrowband LED Illumination[J]. Chinese Journal of Lasers, 2020, 47(12): 1207006

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