Chinese Journal of Lasers, Volume. 49, Issue 20, 2007209(2022)
Cancer Cell Vacuolization Imaging Based on Digital Holographic Microscopy Tomography
Fig. 2. Phase images of SiO2 microsphere at different angles. (a) 90°;(b) 180°;(c) 0°
Fig. 3. Three-dimensional tomographic reconstruction results of SiO2 microsphere. (a) Central slice in x-y plane; (b) central slice in x-z plane; (c) central slice in y-z plane; (d) three-dimensional tomographic reconstruction result of SiO2 microsphere
Fig. 4. Holograms at different angles and at the same position and their corresponding spectra, where the area inside the circle is selected filtering window. (a)(d) 90°;(b)(e) 180°;(c)(f) 0°
Fig. 5. Variation of focusing criterion with focusing distance and corresponding amplitude images of different focusing distances, where rectangle represents the selected focus window. (a) Variation of focusing criterion with focusing distance, focusing distance corresponding to the lowest point of the curve is the best reconstruction distance; (b)-(d) reconstruction amplitude images, focusing distance is -3.00, -1.51, and 0 cm, respectively
Fig. 6. Cell phase images before and after phase unwrapping. (a) Cell phase image before phase unwrapping; (b) cell phase image after unwrapping
Fig. 7. Phase images of bladder cancer cells with vacuoles, phase distribution along the dotted line of phase images, and three-dimensional display results of phase images. (a) Cell 1; (b) cell 2; (c) cell 3; (d) cell 4
Fig. 8. Three-dimensional tomographic reconstruction results of bladder cancer cells with vacuoles. (a) Cell 1; (b) cell 2;(c) cell 3; (d) cell 4
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Yakun Liu, Wen Xiao, Leiping Che, Yinjia Li, Xi Xiao, Hao Wang, Feng Pan. Cancer Cell Vacuolization Imaging Based on Digital Holographic Microscopy Tomography[J]. Chinese Journal of Lasers, 2022, 49(20): 2007209
Category: Biomedical Optical Imaging
Received: May. 20, 2022
Accepted: Aug. 18, 2022
Published Online: Oct. 14, 2022
The Author Email: Pan Feng (panfeng@buaa.edu.cn)