Study on Interaction Between Mitiglinide Calcium and Bovine Serum Albumin by Spectroscopy

Xiaotian Zhang; Lin Zhang; Weihua Zhu; Bo Yu

doi:10.3788/AOS201737.1130002

Acta Optica Sinica, Volume. 37, Issue 11, 1130002(2017)

Study on Interaction Between Mitiglinide Calcium and Bovine Serum Albumin by Spectroscopy

Xiaotian Zhang¹, Lin Zhang^1、*, Weihua Zhu¹, and Bo Yu²

Author Affiliations

¹ College of Science, Hohai University, Nanjing, Jiangsu 211100, China

² College of Science, Nanjing University of Aeronautics and Astronautics, Nanjing, Jiangsu 210016, China

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Figures & Tables(5)

Fig. 1. Quenching spectra of mitiglinide calcium to BSA

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Fig. 2. Stern-Volmer quenching curves of BSA after adding mitiglinide calcium

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Fig. 3. Fluorescence spectrum of BSA and ultraviolet visible absorption spectrum of mitiglinide calcium

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Fig. 4. Synchronous fluorescence spectra of mitiglinide calcium at different wavelength differences. (a) Δλ=15 nm; (b) Δλ=60 nm

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Table 1. Fitting equation, fitting degree R2 and quenching rate constant kq
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Table 1. Fitting equation, fitting degree R2 and quenching rate constant kq
T /K Stern-Volmer linear equation R² $\begin{matrix} {k_{q}}_{} \end{matrix}$ /(10⁵ L·mol^-1)
297 F₀/F=0.9544+8.78×10⁵c_q 0.97538 8.78
308 F₀/F=0.94165+1.304×10⁶c_q 0.99399 13.04

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Xiaotian Zhang, Lin Zhang, Weihua Zhu, Bo Yu. Study on Interaction Between Mitiglinide Calcium and Bovine Serum Albumin by Spectroscopy[J]. Acta Optica Sinica, 2017, 37(11): 1130002

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