Fig. 1. Schematic illustration of a wide-field and GP-SIDH imaging system.

Fig. 2. Imaging schematic for multifunctional GP-SIDH microscopy. (a) The property of the GP lens. (b) The principle of the geometric phase shift. (c) The process of computational reconstruction.

Fig. 3. Schematic of the fluorescence holographic microscope. (a) Experiment setup. (b) Wide-field model images. (c) GP-SIDH model images, captured at different axial positions, respectively.

Fig. 4. Experimental results of fluorescent microspheres and resolution plate target. (a), (c) Recorded holograms, with different phase shifting values. (b) GP-SIDH refocused on different spatial depths. (d) GP-SIDH reconstruction results. (e) Wide-field images at different spatial depths. (f) Wide-field image on the focus. (g), (h) xy and xz intensity distribution of 500 nm microspheres.

Fig. 5. Aberration correction results. (a), (c) Uncorrected microsphere xy and xz plane images. (d), (f) Corrected xy and xz plane images. (b), (e) are the Fourier spectrum of (a), (d). (g) Reconstructed aberrated images. (h) Corrected images. (i) Wide-field images. (g1), (g2) to (i1), (i2) are the amplification of (g)–(i), acting-labeled in U2OS cells. (j) Intensity of underlines, respectively.

Fig. 6. Imaging performance of the multifunctional microscope. (a)–(d) Reconstructed aberrated images, corrected images, edge images, wide-field images. (a1)–(d1) and (a4)–(d4) are the xy and xz intensity distribution. (a2), (a3), (b2), (b3), (c2), (c3), and (d2), (d3) are the amplification of (a1)–(d1), acting-labeled in COS7 cells, respectively.