Glutamate transporter GluA, is a class of ATP-binding proteins that can affect intracellular carbon metabolism, nitrogen metabolism and secondary metabolism by regulating the active transport of glutamate, belonging to the ABC transport system. To study the effects of GluA on the strain growth development and butenyl-spinosyn biosynthesis in Saccharopolyspora pogona, gluA-1 overexpression and deletion strains were constructed by genetic engineering technology. Phenotypic analysis showed that the butenyl-spinosyn production increased by 4.2 times, the bacterial growth logarithmic phase was prolonged, the maximum bacterial density and acetyl-CoA content significantly increased, whereas the mycelium morphology was not significantly changed in overexpressed strain S. pogona::gluA-1. The butenyl-spinosyn production decreased by 37%, the strain growth was inhibited obviously, the acetyl-CoA content significantly decreased, and the mycelium was shortened and thickened, branching was reduced in deletion strain S. pogona-ΔgluA-1. These results indicated that GluA could promote the growth development and the butenyl-spinosyn biosynthesis in S. pogona, which laid an important foundation for the study of GluA's role in the secondary metabolism of Streptomyces.