Opto-Electronic Advances, Volume. 6, Issue 9, 230004(2023)

Rapid inactivation of human respiratory RNA viruses by deep ultraviolet irradiation from light-emitting diodes on a high-temperature-annealed AlN/Sapphire template

Ke Jiang1,2、†, Simeng Liang3、†, Xiaojuan Sun1,2、*, Jianwei Ben1,2, Liang Qu3, Shanli Zhang1,2, Yang Chen1,2, Yucheng Zheng3, Ke Lan3,4, Dabing Li1,2、**, and Ke Xu3,4、***
Author Affiliations
  • 1State Key Laboratory of Luminescence and Applications, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, China
  • 2Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing 100049, China
  • 3State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China
  • 4Institute for Vaccine Research, Animal Biosafety Level 3 Laboratory, Wuhan University, Wuhan 430072, China
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    Figures & Tables(7)
    Cross-sectional BFDB STEM images (<1-100>) of the n-AlGaN epilayer grown on a HTA AlN/Sapphire template. (a, b) and (c, d) are the images without and with SLs, respectively. (a, c) and (b, d) are taken with g = (0002) and (11-20), respectively. For one sample, the images with different g vectors are taken in the same area. The white arrows in (b) and (d) denote the edge dislocations in the HTA AlN/Sapphire template.
    Surface and strain states of the n-AlGaN epilayer grown on a HTA AlN/Sapphire template and structure of the SLs. (a, b) and (c, d) are AFM images (5 μm × 5 μm) and XRD RSMs of the (–105) planes for wafers without/with SLs. (e–h) Cross-sectional HAADF STEM image (<11–20>) of an AlN/AlGaN SLs area and corresponding EDS mappings of N, Al, and Ga elements.
    DUV LED structure grown on a HTA AlN/Sapphire template. (a) Structure diagram of the DUV LED. (b) XRD RSM of the (105) plane of the DUV LED wafer. (c–e) Cross-sectional HAADF STEM images (<11–20>) of the p-AlGaN HIL, MQWs, and EBL (areas 1, 2, and 3 inFig. 3(a)) of the DUV LED, respectively. (f–h) HAADF STEM image (<11–20>) of the DUV LED (area 4 inFig. 3(a)) and corresponding EDS mappings of Al and Ga.
    Performance characterizations of the three DUV LEDs. (a) IV curves. The inset shows the normalized EL spectra at the current of 100 mA. (b) LOP and EQE curves. The inset shows a picture of a typical flip-chip packaged LED. (c) Distance-dependent LPD at the current of 100 mA. The insets show the log-scale graph and measurement geometry.
    Inactivation effects of the DUV LEDs on IAV and SARS-CoV-2. (a) Inactivation efficiency for IAV with an initial titer of 2.3×104 PFUs in 60 μL. The inset shows the results for a higher initial titer of 2.3×105 PFUs in 60 μL. (b) Irradiation time-dependent viral inactivation effects for IAV from 2 to 12 cm. The inset shows the live viral titer after 10 s at 8 and 12 cm. (c) Viral inactivation effects of the 256 nm-LED for IAV in 10 s at 4 cm. The viral titers determined by the ratio of PFUs (bar chart) and log10 reduction (cyan line) are shown. (d) Live viral titer after 10 s irradiation for IAV at 4 cm on different materials. Values are presented as the means±SDs (n=3, n=number of independent replicates). (e) Inactivation efficiency with an initial titer of 2.3×104 PFUs in 60 μL for SARS-CoV-2. Values are presented as the means±SDs (n=2, n=number of biological replicates). (f) Inactivation effects of the 256 nm-LED for different pSARS-CoV-2 variants. BHK21-hACE2 cells are infected for 24 h with pseudo-SARS-CoV-2 irradiated or not, and then, the luciferase activity is measured to reflect the virus entry efficiency. Time-dependent effects at 4 cm are evaluated by measuring the luciferase activity for individual pSARS-CoV-2. The dotted line represents the detection limit. Values are presented as the means±SDs (n=3, n=number of independent replicates). **p<0.01.
    Total relative RNA levels in the cell (a) supernatants and (b) lysates infected by a virus suspension irradiated for 10 s at 4 cm or not after 24 h. The relative RNA level is normalized to that of the mock (non-infected) group. Values are presented as the means±SDs (n=3, n=number of independent replicates). **p < 0.01, ***p < 0.001, ****p < 0.0001.
    • Table 1. Estimation of the inactivation ability of the 256 nm-LED for human respiratory RNA viruses including IAV, SARS-CoV-2, and HPIV. The viral titers of the virus stock in 60 μL are shown. The values represent means±SD of 3 independent experiments for IAV and HPIV and means±SD of 2 independent experiments for SARS-CoV-2.

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      Table 1. Estimation of the inactivation ability of the 256 nm-LED for human respiratory RNA viruses including IAV, SARS-CoV-2, and HPIV. The viral titers of the virus stock in 60 μL are shown. The values represent means±SD of 3 independent experiments for IAV and HPIV and means±SD of 2 independent experiments for SARS-CoV-2.

      VirusVirus original titerExposure time (s)Light power density (mW/cm2)Irradiation dose (mJ/cm2)Reduction ratioIrradiation ability
      IAV3,833,333 (PFU)10~0.8899.99%> 3,820,000 (PFU)
      SARS-CoV-222,500 (PFU)10~0.88100%≥ 22,500 (PFU)
      HPIV106,800 (TCID50)10~0.88100%≥ 106,800 (TCID50)
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    Ke Jiang, Simeng Liang, Xiaojuan Sun, Jianwei Ben, Liang Qu, Shanli Zhang, Yang Chen, Yucheng Zheng, Ke Lan, Dabing Li, Ke Xu. Rapid inactivation of human respiratory RNA viruses by deep ultraviolet irradiation from light-emitting diodes on a high-temperature-annealed AlN/Sapphire template[J]. Opto-Electronic Advances, 2023, 6(9): 230004

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    Paper Information

    Category: Research Articles

    Received: Jan. 23, 2023

    Accepted: Apr. 24, 2023

    Published Online: Nov. 15, 2023

    The Author Email:

    DOI:10.29026/oea.2023.230004

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