Acta Optica Sinica, Volume. 44, Issue 2, 0200003(2024)
Research on High-Precision Quantitative Phase Microscopy Imaging Methods
Fig. 1. Structure diagrams of commonly used DHMs. (a) Transmission DHM; (b) reflection DHM
Fig. 2. Flow chart of reconstruction of DHM. (a) Recorded off-axis hologram; (b) frequency spectrum of hologram and spectrum filtering; (c) reconstructed phase map
Fig. 4. Measurement results of phase-step with common-path simultaneous phase-shifting DHM[36]. (a) Single-shot recorded simultaneous phase-shifting hologram (phase shift between I1 and I2 is π/2); (b) reconstructed phase map of phase-step
Fig. 5. Stability test results for common-path simultaneous phase-shifting DHM[36]
Fig. 6. Schematic of DHM setup with sutructed illumination[41]. (a) Optical setup; (b) four groups of fringe patterns loaded in SLM; (c) frequency spectrum of generated hologram with sutructed illumination
Fig. 7. Experimental results of DHM with structed illumination[41]. (a) Recorded off-axis hologram; (b) reconstructed phase image using plane wave illumination; (c) reconstructed phase image using structured illumination; (d) phase distributions along two lines drawn in (b) and (c)
Fig. 8. Experimental results of grating topography of DHM with dual-wavelenth illumination[45]. (a) Recorded dual-wavelength in-line hologram; (b), (c) red and blue light holograms extracted from (a); (d) reconstructed topography of grating; (e) comparison of in-line and off-axis holographic measurement results
Fig. 10. Comparison of experimental results on phase-step sample[47]. (a) Recorded hologram with LED illumination (area inside white rectangular box is enlarged displayed); (b) Spatial spectral distribution of (a) (circle dashed box represents spectral filtering range); (c) reconstructed height distribution with LED illumination; (d) recorded hologram with He-Ne laser illumination; (e) reconstructed height distribution reconstructed with He-Ne laser illumination; (f) height profiles at cross sections marked with dashed lines in (c) and (e)
Fig. 11. Experimental results for autofocusing of DHM basd on structured illumination[41]. (a) Focus criterions by amplitude variation (VAR) and proposed method; (b) reconstructed phase image with optimal focusing distance (Δz=3.2 cm)
Fig. 12. Experimental results of autofocusing using dual-wavelength diffraction difference method[49]. (a)-(c) Differences between dual-wavelength reconstructed images with Δz=0, 7.5, and 15 cm, respectively; (d) reconstructed phase distribution of specimen for red light with Δz=15 mm; (e) comparison of evaluation function curves between different autofocusing methods
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Junwei Min, Peng Gao, Dan Dan, Juanjuan Zheng, Xianghua Yu, Baoli Yao. Research on High-Precision Quantitative Phase Microscopy Imaging Methods[J]. Acta Optica Sinica, 2024, 44(2): 0200003
Category: Reviews
Received: Jun. 27, 2023
Accepted: Aug. 4, 2023
Published Online: Jan. 11, 2024
The Author Email: Yao Baoli (yaobl@opt.ac.cn)
CSTR:32393.14.AOS231191