Chinese Optics Letters, Volume. 18, Issue 10, 101701(2020)

Automated superpixels-based identification and mosaicking of cone photoreceptor cells for adaptive optics scanning laser ophthalmoscope

Yiwei Chen1, Yi He1, Jing Wang1,2, Wanyue Li1,2, Lina Xing1, Feng Gao1, and Guohua Shi1,2,3、*
Author Affiliations
  • 1Jiangsu Key Laboratory of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China
  • 2Department of Biomedical Engineering, University of Science and Technology of China, Hefei 230041, China
  • 3Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai 200031, China
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    Figures & Tables(8)
    Diagram depicting the image processing of the proposed algorithmic steps.
    Example of image denoising: (a) before denoising and (b) after denoising.
    Cone photoreceptor cell number estimation: (a) denoised image, (b) power of discrete Fourier transform (DFT) of a log10 compressed, (c) averaged slice of (b), fitted curve in red, and (d) subtraction outcome of fitted curve (highlighted in red) from the blue curve in (c).
    Simple linear iterative clustering (SLIC) superpixels segmentation: (a) original image patch and (b) segmented image with oversegmentation.
    Superpixels merging process.
    Example of superpixels merging outcome: (a) before merging and (b) after merging.
    Performance of the proposed method: (a) input AO-SLO image, (b) identification of cells and segmented image, and (c) mosaic image.
    • Table 1. Evaluation of the Effectiveness of Cell Identification: Overall Precision, Recall, and F1-Score Outcomes

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      Table 1. Evaluation of the Effectiveness of Cell Identification: Overall Precision, Recall, and F1-Score Outcomes

      PrecisionPercentage of actual cells in identified cells77.3%
      RecallPercentage of actual cells identified95.2%
      F1-score2×Precision×Recall/(Precision+Recall)85.3%
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    Yiwei Chen, Yi He, Jing Wang, Wanyue Li, Lina Xing, Feng Gao, Guohua Shi, "Automated superpixels-based identification and mosaicking of cone photoreceptor cells for adaptive optics scanning laser ophthalmoscope," Chin. Opt. Lett. 18, 101701 (2020)

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    Paper Information

    Category: Biomedical Optics

    Received: Mar. 21, 2020

    Accepted: Jun. 1, 2020

    Published Online: Aug. 5, 2020

    The Author Email: Guohua Shi (ghshi_lab@126.com)

    DOI:10.3788/COL202018.101701

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