Chinese Optics, Volume. 16, Issue 5, 1010(2023)
Research progress of miniature head-mounted single photon fluorescence microscopic imaging technique
Fig. 1. A system with a basic imaging function. (a) Cross sectional view of integrated microscope proposed by Ghosh
Fig. 2. A system with wiress function. (a) Cross sectional view of FinchScope; (b) internal optics element layout of wireless miniscope
Fig. 3. A system with 3D imaging functionality. (a) Cross sectional view of MiniLFM; (b) cross sectional view of Miniscope3D; (c) microscope cross section by Bagramyan
Fig. 4. A system with dual region imaging functionality. (a) NINscope body and internal optics element layout; (b) a mouse with two NINscopes mounted
Fig. 5. A system with two-color imaging functionality. (a) Cross sectional view of MiniScope V4; (b) cross sectional view of DCFIMM-SBI; (c) cross sectional view of DCFIMM-DBI
Fig. 6. Existing large filed of view system. (a) Imaging optical path of cScope; (b) imaging optical path of CM2; (c) fully assembled mScope
Table 1. Optical system and optical performance parameters of the miniature fluorescence microscope with basic imaging functionality
View tableView in ArticleTable 1. Optical system and optical performance parameters of the miniature fluorescence microscope with basic imaging functionality
系统参数 Ghosh 等人 MiniScope V3 miniscope CHEndoscope Bagramyan 等人 物镜 梯度折射率透镜 梯度折射率透镜 非球面透镜 梯度折射率透镜 梯度折射率透镜 管镜 双胶合透镜 双胶合透镜 双胶合透镜 双胶合透镜 平凸透镜 视场 600 μm×800 μm 750 μm×450 μm 1100 μm×1100 μm ~500 μm ~105 μm 分辨率 2.5 μm 1.0 μm/pix 单细胞分辨率 单细胞分辨率 1.0 μm 图像传感器 MT9V021 (5.6 μm/pix) MT9V032 (6.0 μm/pix) MT9V022 (6.0 μm/pix) MT9P031 (2.2 μm/pix) OV7251 (3.0 μm/pix) 成像速度 36 Hz 60 Hz 10 Hz 20 Hz 50 Hz
Table 2. Optical system and optical performance parameters of a miniature fluorescence microscope with wireless function
View tableView in ArticleTable 2. Optical system and optical performance parameters of a miniature fluorescence microscope with wireless function
系统参数 FinchScope Wire-free MiniScope miniscope wScope 物镜 梯度折射率透镜 梯度折射率透镜 非球面透镜 梯度折射率透镜 管镜 双胶合透镜 双胶合透镜 双胶合透镜 双胶合透镜 视场 800 μm×600 μm — 500 μm×500 μm 700 μm×450 μm 分辨率 单细胞分辨率 1 μm/pix 单细胞分辨率 1.8 μm 图像传感器 OV7960(6.00 μm/pix) EV76C454(5.80 μm/pix) MT9V022(6.00 μm/pix) OV7690 (1.75 μm/pix) 成像速度 30 Hz 10 Hz 10 Hz 25 Hz
Table 3. Optical system and optical performance parameters of the miniature fluorescence microscope with 3D imaging functionality
View tableView in ArticleTable 3. Optical system and optical performance parameters of the miniature fluorescence microscope with 3D imaging functionality
系统参数 MiniLFM Miniscope3D Bagramyan等人 OMKAR 等人 物镜 梯度折射率透镜 梯度折射率透镜 梯度折射率透镜 两片双胶合透镜 管镜 双胶合透镜 相位掩模板 平凸透镜 双胶合透镜 视场 700 μm×600 μm×360 μm 900 μm×700 μm×390 μm 横向 150 μm 轴向 98 μm 横向207 μm 轴向220 μm 三维成像元件 微透镜阵列 相位掩模板 可调谐液晶透镜 电湿润透镜 横向分辨率 6.0 μm 2.8 μm 1.4 μm 1.0 μm/pix 轴向分辨率 30.0 μm 15.0 μm 15.0 μm 18.0 μm 图像传感器 MT9V032 (6.0 μm/pix) MT9V032 (6.0 μm/pix) OV7251 (3.0 μm/pix) MT9P031 (2.2 μm/pix) 成像速度 16 Hz 40 Hz 50 Hz —
Table 4. Optical system and optical performance parameters of a miniature fluorescence microscope with dual region imaging functionality
View tableView in ArticleTable 4. Optical system and optical performance parameters of a miniature fluorescence microscope with dual region imaging functionality
系统参数 Gonzalez 等人 NINscope 物镜 梯度折射率透镜 梯度折射率透镜 管镜 双胶合透镜 平凸透镜 视场 600 μm×479 μm 786 μm×502 μm 分辨率 0.83 μm/pix 单细胞分辨率 图像传感器 OV7690 (6 μm/pix) PYTHON480 (4.8 μm/pix) 成像速度 — 30 Hz
Table 5. Optical system and optical performance parameters of a miniature fluorescence microscope with two-color imaging functionality
View tableView in ArticleTable 5. Optical system and optical performance parameters of a miniature fluorescence microscope with two-color imaging functionality
系统参数 MiniScope V4 DCFIMM-SBI DCFIMM-DBI 物镜 两片双胶合透镜 两片双胶合透镜 双胶合透镜 管镜 双胶合透镜 双胶合透镜 双胶合透镜 视场 ~1.00 mm2 1.10 mm×1.10 mm 0.77 mm×0.77 mm 分辨率 单细胞分辨率 3.47 μm 3.47 μm 图像传感器 PYTHON480 (4.8 μm/pix) EV76C454 (5.8 μm/pix) EV76C454 (5.8 μm/pix) 成像速度 120 Hz 20 Hz 20 Hz
Table 6. Optical system composition and optical performance parameters of miniature single photon fluorescence microscope with a small field
View tableView in ArticleTable 6. Optical system composition and optical performance parameters of miniature single photon fluorescence microscope with a small field
系统参数 小视场系统 (FOV<1mm) 物镜 梯度折射率透镜[ 9 -10 ,15 -17 ,21 ,23 -24 ,27 -28 ,30 -31 ] 、双胶合透镜[ 29 ,32 ,35 ] 、非球面透镜 [12 ,22 ]管镜 双胶合透镜[ 9 -10 ,12 ,15 ,17 ,21 -24 ,29 -30 ,32 ,35 ] 、平凸透镜[ 16 ,28 ,31 ] 、相位掩模板[27 ]分辨率范围 最小：1 μm[ 16 ] , 最大：6 μm[24 ]重量范围 最小：1.3 g[ 16 ] , 最大：6.7 g[29 ]
Table 7. Optical system and optical performance parameters of a large field miniature fluorescence microscope
View tableView in ArticleTable 7. Optical system and optical performance parameters of a large field miniature fluorescence microscope
cScope CM2 mScope 物镜 多片球面透镜 微透镜阵列 双凸透镜 管镜 多片球面透镜 视场 7.8 mm×4.0 mm 7.3 mm×8.1 mm× 2.5 mm 8.0 mm×10.0 mm 横向分辨率 14.0 μm 7 μm 39.4~55.7 μm 图像传感器 MT9V032 (6 µm/pix) MT9P031 (2.2 µm/pix) MT9V032 (6 µm/pix) 成像速度 60Hz — —
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Qiang FU, Zhi-miao ZHANG, Shang-nan ZHAO, Yang LIU, Yang DONG. Research progress of miniature head-mounted single photon fluorescence microscopic imaging technique[J]. Chinese Optics, 2023, 16(5): 1010
Category: Review
Received: Jan. 10, 2023
Accepted: Mar. 24, 2023
Published Online: Oct. 27, 2023
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