Laser & Optoelectronics Progress, Volume. 57, Issue 9, 091801(2020)

Effect of Optical System on the Fluorescence Depletion Pattern in the Parallelized STED Microscopy

Shuochen Zhang and Jihong Feng*
Author Affiliations
  • Intelligent Physiological Measurement and Clinical Translation, Beijing International Base for Scientific and Technological Cooperation, College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100024, China
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    Figures & Tables(3)
    Parallelized STED simplified perfect optical system model of microscopic imaging system
    Theoretical calculation results. (a)Derived function of parallelized fluorescence depletion patterns periodicity dP/du with inclination angle of object space u; (b) parallelized fluorescence depletion patterns periodicity P and the optimum tube lens focal length foptimum with inclination angle of object space u
    Simulation results of parallelized fluorescence depletion patterns. (a) Interference patterns produced by horizontal polarization STED beams in focus; (b)interference patterns produced by vertical polarization STED beams in focus; (c) parallelized fluorescence depletion patterns produced by all STED beams in focus
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    Shuochen Zhang, Jihong Feng. Effect of Optical System on the Fluorescence Depletion Pattern in the Parallelized STED Microscopy[J]. Laser & Optoelectronics Progress, 2020, 57(9): 091801

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    Paper Information

    Category: Microscopy

    Received: Sep. 17, 2019

    Accepted: Sep. 26, 2019

    Published Online: May. 6, 2020

    The Author Email: Jihong Feng (jhfeng@bjut.edu.cn)

    DOI:10.3788/LOP57.091801

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