Journal of Atmospheric and Environmental Optics, Volume. 19, Issue 1, 38(2024)

A subsection control method of microfluidic automatic sampling in algal cell microscopic imaging

CHEN Zhihao1,2,3, ZHAO Nanjing2,3、*, YIN Gaofang2,3, MA Mingjun1,2,3, DONG Ming1,2,3, HUA Hui1,2,3, and DING Zhichao1,2,3
Author Affiliations
  • 1School of Environmental Science and Optoelectronic Technology, University of Science and Technology of China,Hefei 230026, China
  • 2Key Laboratory of Environment Optics and Technology, Anhui Institute of Optics and Fine Mechanics, HFIPS,Chinese Academy of Sciences, Hefei 230031, China
  • 3Key Laboratory of Optical Monitoring Technology for Environment, Anhui Province , Hefei 230031, China
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    Figures & Tables(10)
    Schematic diagram of experiment system
    Schematic diagram of cell detection device. (a) Binarized fluorescence signal; (b) fluorescence signal of algal cell;(c) detection process of algal cell fluorescence
    Schematic diagram of Injection segment control
    Fluorescence signal of single cell
    Probability distribution of particle velocity passing through a section in unit time
    Velocity distribution of algae cells under different volumetric flow rates and fitted curve.(a) 10 μL/min; (b) 20 μL/min; (c) 30 μL/min; (d) 40 μL/min
    Automatic acquisition of algae cell images at different flow rates and microscopy results. (a) 0.24 μL/min;(b) 1.50 μL/min; (c) 6.00 μL/min; (d) images obtained through microscope
    • Table 1. Comparison of sample average flow rate and theoretical rate results

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      Table 1. Comparison of sample average flow rate and theoretical rate results

      Flow rate/(μL·min-1)5102030406080
      Theoretical velocity/(mm·s-1)1.352.715.418.1210.8316.2421.65

      Experimental

      Velocity/(mm·s-1)

      1.112.765.197.8110.1812.1427.21
      Deviation/%18.001.803.983.775.9625.2525.68
    • Table 2. Comparision of image quality

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      Table 2. Comparision of image quality

      ImageFig.7 (a)Fig.7 (b)Fig.7 (c)Fig.7 (d)
      Velocity/(μL·min-1)0.241.506.000.00
      Average gradient value14.423.161.2114.61
      Deviation/%1.3178.3491.750.00
    • Table 3. Comparison of injection times between segmented control and slow injection

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      Table 3. Comparison of injection times between segmented control and slow injection

      Group12345678
      Injection time under segmented control/s61.0058.00173.0036.0076.6785.00146.00156.33
      115.6737.00131.6771.3340.0096.67102.33128.00
      51.3324.3323.33108.6755.67114.3334.67166.33
      Average time under segmented control/s87.22
      Theoretical slow injection time/s275.00
      Improvement/%68.28
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    Zhihao CHEN, Nanjing ZHAO, Gaofang YIN, Mingjun MA, Ming DONG, Hui HUA, Zhichao DING. A subsection control method of microfluidic automatic sampling in algal cell microscopic imaging[J]. Journal of Atmospheric and Environmental Optics, 2024, 19(1): 38

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    Paper Information

    Category:

    Received: Apr. 8, 2021

    Accepted: --

    Published Online: Mar. 19, 2024

    The Author Email: Nanjing ZHAO (njzhao@aiofm.ac.cn)

    DOI:10.3969/j.issn.1673-6141.2024.01.003

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