Fig. 1. The single slice bimodal images of the decolorization of hydrochloric acid ethanol after HE staining of paraffin slices of mouse renal tissue. (a), (e) and (i) are HE staining images. (b)~(d) are the same field of view immunofluorescence images of EDTA antigen retrieval after de-colorizing in Fig.(a). (f)~(h) are the immunofluorescence images of the same field of view of Tris-EDTA antigen retrieval after the decolorization of Fig.(b). (j)~(l) are the same field of view immunofluorescence images of citric acid antigen retrieval after de-colorizing in Fig.(i). (m) is the statistical analysis of the signal-to-noise ratio of immunofluorescence images under different antigen retrieval conditions after hydrochloric acid ethanol decolorization. Scale: 50 μm

Fig. 2. The single-slice bimodal images of glacial acetic acid and oxalic acid decolorization after HE staining of paraffin slices of mouse renal tissue. (a), (e) and (i) are HE staining images, and (b)~(d) are the same field of view immunofluorescence images of EDTA antigen retrieval after de-colorizing in Fig.(a). (f)~(h) are the immunofluorescence images of the same field of view of Tris-EDTA antigen retrieval after the decolorization of Fig.(b). (j)~(l) are the same field of view immunofluorescence images of citric acid antigen retrieval after de-colorizing in Fig.(i). (m) is the statistical analysis of the signal-to-noise ratio of immunofluorescence images under different antigen retrieval conditions after glacial acetic acid-oxalic acid decolorization. Scale: 50 μm

Fig. 3. The single-slice bimodal images of potassium permanganate-oxalic acid decolorization after HE staining of paraffin slices of mouse renal tissue. (a), (e), (i) are HE staining. (b)~(d) are the same field of view immunofluorescence images of EDTA antigen retrieval after de-colorizing in Fig.(a). (f)~(h) are the immunofluorescence images of the same field of view of Tris-EDTA antigen retrieval after de-colorizing in Fig.(b). (j)~(l) are the same field of view immunofluorescence images of citric acid antigen retrieval after de-colorizing in Fig.(i). (m) is the statistical analysis of the signal-to-noise ratio of immunofluorescence images under different antigen repair conditions after potassium permanganate-oxalic acid decolorization. Scale: 50 μm

Fig. 4. The single-slice bimodal images of the mouse renal tissue de-colorized with glacial acetic acid and oxalic acid and repaired with EDTA, after HE staining. (a) is HE staining image obtained under a 60 × microscope. (b) is an immunofluorescence image of the renal collecting duct labeled with AQP-2 and DAPI in the same field of view. (c) is the fusion image of HE and immunofluorescence