Bright-field imaging can provide cellular and histological morphological information, while fluorescence imaging can provide expression information of key proteins. Dual-mode correlation imaging that combines both techniques is used for examining tissue samples in medical and scientific research. However, in clinical, correlation imaging often relies on adjacent tissue sections for observation. This can result in inconsistencies at both the tissue structure and the cellular level, which is problematic when the sample volume is limited, the number of cells on the slices is sparse, or precise point-to-point morphological information is required. In this study, we present a sample preparation method that enables both Hematoxylin and Eosin (H&E) staining and immunofluorescence staining on a single tissue section, enabling dual-mode imaging. We focused on optimizing the decolorizing process and the immunofluorescence restaining protocol, comparing three decolorization methods (hydrochloric acid ethanol, acetic acid-oxalic acid, and potassium permanganate-oxalic acid) and three antigen retrieval methods (EDTA, Tris-EDTA, and citric acid). By analyzing the signal-to-noise ratios (SNR) of images obtained under different conditions, we found that the combination of acetic acid-oxalic acid decolorization with EDTA antigen retrieval produced the best quality immunofluorescence images. Additionally, we successfully fused brightfield and fluorescence images, providing more comprehensive tissue morphology and immunological information on a single section.