Acta Laser Biology Sinica, Volume. 33, Issue 3, 209(2024)

Segmentation and Quantification of Zebrafish Hindbrain Vessel ConfocalImages Based on DE-3D U-Net

ZHENG Wenhu1, LI Hui2、*, CHEN Chong2, and WANG Linbo2
Author Affiliations
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  • 2[in Chinese]
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    References(27)

    [1] [1] O’BROWN, PFAU S J, GU C. Bridging barriers: a comparativelook at the blood-brain barrier across organisms[J]. Genes & Development, 2018, 32(7/8): 466-478.

    [2] [2] ANDREONE B J, CHOW B W, TATA A, et al. Blood-brain barrierpermeability is regulated by lipid transport-dependent suppression ofcaveolae-mediated transcytosis[J]. Neuron, 2017, 94(3): 581-582.

    [3] [3] CHO C, SMALLWOOD P M, NATHANS J. Reck and GPR124 areessential receptor cofactors for Wnt7a/Wnt7b-specific signaling inmammalian CNS angiogenesis and blood-brain barrier regulation[J]. Neuron, 2017, 95(5): 1065-1066.

    [4] [4] ABBOTT N N J, ABBOTT A A K, PATABENDIGE D E, et al.Structure and function of the blood-brain barrier[J]. Neurobiology of Disease, 2010, 37(1): 13-25.

    [5] [5] ARISS M M, TERRY A R, HAY N, et al. Amalgam regulates thereceptor tyrosine kinase pathway through sprouty in glial cell development in the drosophila larval brain[J]. Journal of Cell Science, 2020, 133(19): 24-37.

    [6] [6] AUGUSTIN H G, KOH G Y. Organotypic vasculature: from descriptive heterogeneity to functional pathophysiology[J]. Science,2017, 357(6353): 2379-2379.

    [7] [7] ARTUS C, GLACIAL F, GANESHAMOORHY K, et al. The Wnt/planar cell polarity signaling pathway contributes to the integrityof tight junctions in brain endothelial cells[J]. Journal of CerebralBlood Flow and Metabolism, 2014, 34(3): 433-440.

    [8] [8] BATS M L, PEGHAIRE C, DELOBEL V, et al. Wnt/frizzled Signaling in endothelium: a major player in blood-retinal- and bloodbrain-barrier integrity[J]. Cold Spring Harbor Perspectives inMedicine, 2022, 12(4): 1-12.

    [9] [9] ANDREONE B J, CHOW B W, TATA A, et al. Blood-brain arrierpermeability is regulated by lipid transport-dependent suppression of caveolae-mediated transcytosis[J]. Neuron, 2017, 94(3):581-582.

    [10] [10] BILL B R, KORZH V. Choroid plexus in developmental and evolutionary perspective[J]. Frontiers in Neuroscience, 2014, 8(6): 53-64.

    [11] [11] GUALDA E J, PEREIRA H, VALE T, et al. SPIM-fluid: opensource light-sheet based platform for high-throughput imaging[J].Biomedical Optics Express, 2015, 6(11): 4447-4456.

    [12] [12] GARCIA-LECEA M, KONDRYCHYN I, FONG S H, et al. In vivo analysis of choroid plexus morphogenesis in zebrafish[J].PLoS One, 2008, 3(9): 1-13.

    [13] [13] VAN LEEUWEN L M, EVANS R J, JIM K K, et al. A transgeniczebrafish model for the in vivo study of the blood and choroid plexusbrain barriers using claudin 5[J]. Biology Open, 2018, 7(2): 1-9.

    [14] [14] DAEWYLER S, FIOLKA R P. Light-sheets and smart microscopy,an exciting future is dawning[J]. Communications Biology, 2023,6(1): 17-29.

    [15] [15] DAMKIER H H, PRAETORIUS J. Genetic ablation of Slc4a10alters the expression pattern of transporters involved in solutemovement in the mouse choroid plexus[J]. American Journal ofPhysiology-Cell Physiology, 2012, 302(10): C1452-C1459.

    [16] [16] ARDESTANI S B, MATCHKOV V V, PRAETORIUS J. Microelectrodes for measurement of intracellular K+ and Na+ in thechoroid plexus epithelium and cerebrospinal fluid[J]. The FASEBJournal, 2022, 36(1): 1-12.

    [17] [17] DAETWLER S, GUENTHER U, MODES C D, et al. Multi-sample SPIM image acquisition, processing and analysis of vasculargrowth in zebrafish[J]. Development, 2019, 146(6): 17-29.

    [18] [18] DAMKIER H H, PRASAD V, HUEBNER C A, et al. Nhe1 isa luminal Na+/H+ exchanger in mouse choroid plexus and is targeted to the basolateral membrane in Ncbe/Nbcn2-null mice[J].American Journal of Physiology-Cell Physiology, 2009, 296(6):C1291-C1300.

    [19] [19] ISOGAI S, HORIGUCHI M, WEINSTEIN B M. The vascularanatomy of the developing zebrafish: an atlas of embryonic andearly larval development[J]. Developmental Biology, 2001,230(2): 278-301.

    [20] [20] COTTARELLI A, CORADA M, BEANOUSSENKO G V, et al.Fgfbp1 promotes blood-brain barrier development by regulatingcollagen IV deposition and maintaining Wnt/beta-catenin signaling[J]. Development, 2020, 147(16): 12-25.

    [21] [21] FETSKO A R, SEBO D J, TAYLOR M R. Brain endothelial cellsacquire blood-brain barrier properties in the absence of Vegfdependent CNS angiogenesis[J]. Developmental Biology, 2023,49(4): 46-59.

    [22] [22] BENNETT D V, AHRENS M B. A practical guide to light mheet microscopy[J]. Methodsin Molecular Biology, 2016, 1451(51): 321-331.

    [23] [23] CAI Y, CHEN Y, XIA Y, et al. Single-lens light-sheet fluorescencemicroscopy based on micro-mirror array[J]. Laser & PhotonicsReviews, 2022, 16(8): 1-7.

    [24] [24] BENNETT D V, AHRENS M B. A practical guide to light sheetmicroscopy[J]. Methods in Molecular Biology, 2016, 1451(51):321-331.

    [25] [25] OSIBOTE O A, DENDERE R, KRISHNAN S, et al. Automatedfocusing in bright-field microscopy for tuberculosis detection[J].Journal of Microscopy, 2010, 240(2): 155-163.

    [26] [26] CALISESI G, CANDEO A, FARINA A, et al. Three-dimensionalbright-field microscopy with isotropic resolution based on multiview acquisition and image fusion reconstruction[J]. ScientificReports, 2020, 10(1): 1-9.

    [27] [27] GAO P F, LEI G, HUANG C Z. Dark-field microscopy: recent advances in accurate analysis and emerging applications[J]. Analytical Chemistry, 2021, 93(11): 4707-4726.

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    ZHENG Wenhu, LI Hui, CHEN Chong, WANG Linbo. Segmentation and Quantification of Zebrafish Hindbrain Vessel ConfocalImages Based on DE-3D U-Net[J]. Acta Laser Biology Sinica, 2024, 33(3): 209

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    Paper Information

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    Received: Nov. 20, 2023

    Accepted: --

    Published Online: Aug. 14, 2024

    The Author Email: Hui LI (hui.li@sibet.ac.cn)

    DOI:10.3969/j.issn.1007-7146.2024.03.003

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