Acta Optica Sinica, Volume. 43, Issue 5, 0509001(2023)

Microchannel Detection Based on Dual-Wavelength Image-Plane Digital Holographic Microscopy

Siqin Tao, Ming Kong, Wei Liu*, Jianan Xu, Fuxia Cheng, Kaixuan Liu, and Zeqiu Yang
Author Affiliations
  • College of Metrology and Measurement Engineering, China Jiliang University, Hangzhou 310018, Zhejiang, China
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    Figures & Tables(13)
    Schematic of experimental apparatus
    Magnification calibration experimental results. (a) Test target 1951 USAF pattern; (b) element intensity image of group 6; (c) locally magnified intensity image of the 5th pair of lines
    Schematic of micro-nano one-dimensional grid structure
    Experimental results of transverse resolution calibration. (a) Reproduction phase of group 7 element in the resolution target; (b) phase reproduction of grid with 5 μm line width; (c) phase image reproduction of grid with 1 μm line width
    Measurement results of segment difference standard film. (a) Depth image; (b) height graph
    Chip physical diagram and structure diagram. (a) PDMS chip physical diagram; (b) PDMS chip channel at 50× magnification; (c) enlargement structure diagram of silicon substrate chip channel
    Straight channel holograms under different wavelengths. (a) 632.8 nm channel hologram; (b) 632.8 nm reference hologram; (c) 635.32 nm channel hologram; (d) 635.32 nm reference hologram
    Wrapped phase images of straight channel. (a) Wrapped phase for 632.8 nm; (b) wrapped phase for 635.32 nm; (c) equivalent wavelength phase distribution
    Straight channel measurement results. (a) Depth image; (b) three-dimensional topography image; (c) compare experimental result
    Circular chamber measurement results. (a) Depth image; (b) three-dimensional topography image; (c) sectional graph
    Silicon substrate chip measurement results. (a) Depth image; (b) three-dimensional topography image; (c) sectional graph
    • Table 1. Comparison of three-dimensional topography measurement results of microfluidic chip straight channel

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      Table 1. Comparison of three-dimensional topography measurement results of microfluidic chip straight channel

      Measurement methodWidth /μmDepth /μm
      Dual-wavelength digital holography75.848.6
      White light interferometry78.750.0
    • Table 2. Comparison of three-dimensional topography measurement results of circular chamber channels in microfluidic chips

      View table

      Table 2. Comparison of three-dimensional topography measurement results of circular chamber channels in microfluidic chips

      Measurement methodWidth /μmDepth /μm
      Dual-wavelength digital holography76.648.5
      White light interferometry78.950
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    Siqin Tao, Ming Kong, Wei Liu, Jianan Xu, Fuxia Cheng, Kaixuan Liu, Zeqiu Yang. Microchannel Detection Based on Dual-Wavelength Image-Plane Digital Holographic Microscopy[J]. Acta Optica Sinica, 2023, 43(5): 0509001

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    Paper Information

    Category: Holography

    Received: Jul. 27, 2022

    Accepted: Oct. 8, 2022

    Published Online: Mar. 13, 2023

    The Author Email: Liu Wei (liuw@cjlu.edu.cn)

    DOI:10.3788/AOS221540

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