Adaptive Method of Excitation Light Intensity Based on TPLIF Technology for Fast and Accurate Measurement of Photosynthetic Fluorescence Parameters

Xiang Wang; Gaofang Ying; Nanjing Zhao; Tingting Gan; Zhisong Qin; Min Chen; Hui Hua; Ming Dong; Zhichao Ding; Peilong Qi; Lu Wang; Deshuo Meng; Jianguo Liu

doi:10.3788/AOS202040.2412001

Acta Optica Sinica, Volume. 40, Issue 24, 2412001(2020)

Adaptive Method of Excitation Light Intensity Based on TPLIF Technology for Fast and Accurate Measurement of Photosynthetic Fluorescence Parameters

Xiang Wang^1,2, Gaofang Ying^1、*, Nanjing Zhao¹, Tingting Gan¹, Zhisong Qin^1,3, Min Chen^1,2, Hui Hua^1,2, Ming Dong^1,2, Zhichao Ding^1,2, Peilong Qi^1,2, Lu Wang^1,2, Deshuo Meng¹, and Jianguo Liu¹

Author Affiliations

¹Key Laboratory of Environment Optics and Technology, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei, Auhui 230031, China

²University of Science and Technology of China, Hefei, Auhui 230026, China

³School of Computer and Information Security, Guilin University of Electronic Technology, Guilin, Guangxi 541004, China

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Figures & Tables(6)

Fig. 1. Structure of TPLIF measurement system

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Fig. 2. Relationship between E_σ and proportion of closed RCII

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Fig. 3. Flowchart of excitation light intensity adaptive algorithm

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Fig. 4. Fluorescence parameter σ_PSII of different algae

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Fig. 5. Measured F_v/F_m by TPLIF technology and Fast-Ocean. (a) Measured F_v/F_m; (b) corresponding relationship of F_v/F_m

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Table 1. Adaptive results of excitation light intensity of seven species of algae

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Table 1. Adaptive results of excitation light intensity of seven species of algae

Alga	Initialintensity	Firstadjustment	Secondadjustment	Thirdadjustment	Fourthadjustment	Averageintensity	RSD /%
Chlorella pyrenoidosa	6000	19071	19083	19002	19748	19226	1.80
Chlorella vulgaris	6000	20875	19758	20693	20192	20380	2.50
Scenedesmus obliquus	32000	21187	21776	21546	22237	21687	2.00
Microcystis aeruginosa	6000	14012	14098	14563	13974	14162	1.90
Anabaena sp.	6000	13142	13187	13422	12880	13158	1.70
Merismopedia	22000	14931	14755	15536	15000	15056	2.20
Cyclotella sp.	6000	27565	27454	28173	26970	27541	1.80

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Xiang Wang, Gaofang Ying, Nanjing Zhao, Tingting Gan, Zhisong Qin, Min Chen, Hui Hua, Ming Dong, Zhichao Ding, Peilong Qi, Lu Wang, Deshuo Meng, Jianguo Liu. Adaptive Method of Excitation Light Intensity Based on TPLIF Technology for Fast and Accurate Measurement of Photosynthetic Fluorescence Parameters[J]. Acta Optica Sinica, 2020, 40(24): 2412001

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Paper Information

Category: Instrumentation, Measurement and Metrology

Received: Jul. 31, 2020

Accepted: Sep. 15, 2020

Published Online: Nov. 23, 2020

The Author Email: Ying Gaofang (gfyin@aiofm.ac.cn)

DOI:10.3788/AOS202040.2412001

Topics

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Instrumentation, Measurement and Metrology