Laser & Optoelectronics Progress, Volume. 61, Issue 20, 2011022(2024)

Structured Illumination Super-Resolution Fluorescence and Rotating Coherent Scattering Dual-Mode Imaging

Wei Sun1, Gengliang Liu1, Gang Wen2, Xiaohu Chen2, Yong Liang2、**, and Hui Li2、*
Author Affiliations
  • 1School of Biomedical Engineering (Suzhou), Division of Life Science and Medicine, University of Science and Technology of China, Suzhou 215163, Jiangsu , China
  • 2Jiangsu Key Laboratory of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, Jiangsu , China
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    The super-resolution fluorescence imaging method can observe the nanoscale structure of different subcellular organelles, and its specificity comes from fluorescence labeling. The label free imaging method avoids the possible impact of staining process on the sample itself, but loses specificity. Therefore, the two have complementary properties, each with its unique advantages and application scope. Integrating two different imaging technologies into the same instrument can broaden its application scenarios. A dual-mode microscopy imaging system was constructed by combining structured light illumination super-resolution microscopy (SIM) and rotational coherent scattering microscopy imaging (ROCS), which can switch between the two modes at will. The system was tested with fluorescent microsphere standard sample and PANC-1 pancreatic cancer cell sample. The spatial resolution of 111 nm and 145 nm were obtained in SIM and ROCS channels respectively, and the imaging speed could reach about 100 Hz. This system has high spatiotemporal resolution fluorescence and label free imaging functions, providing more convenience and possibilities for the research of cell biology.

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    Wei Sun, Gengliang Liu, Gang Wen, Xiaohu Chen, Yong Liang, Hui Li. Structured Illumination Super-Resolution Fluorescence and Rotating Coherent Scattering Dual-Mode Imaging[J]. Laser & Optoelectronics Progress, 2024, 61(20): 2011022

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    Paper Information

    Category: Imaging Systems

    Received: Apr. 30, 2024

    Accepted: Jun. 17, 2024

    Published Online: Nov. 5, 2024

    The Author Email: Yong Liang (liangy@sibet.ac.cn), Hui Li (hui.li@sibet.ac.cn)

    DOI:10.3788/LOP241204

    CSTR:32186.14.LOP241204

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