Chinese Optics Letters, Volume. 23, Issue 4, 041701(2025)

Method for measuring retinal capillary blood flow velocity by encoded OCTA

Shujiang Chen1...2, Kaixuan Hu1,2, Wei Yi1,2, Fuwang Wu1,2, Yi Wan1,2, Lei Zhang1,2, Jianmei Li1,2, Aiqun Wang1,2, and Weiye Song12,* |Show fewer author(s)
Author Affiliations
  • 1School of Mechanical Engineering, Shandong University, Jinan 250061, China
  • 2Key Laboratory of High Efficiency and Clean Mechanical Manufacture of Ministry of Education, Shandong University, Jinan 250061, China
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    Figures & Tables(7)
    The high-speed OCTA system. SLD, super luminescent diode; FC, fiber coupler; PC, polarization controller; L1, L2, collimating lenses; ND, neutral density filter; DC, dispersion controller; M, mirror; DS, displacement slide; FL, focus tunable lens; GM, two-dimensional scanning galvanometer; L3, achromatic doublet lens; L4, two achromatic doublet lens; AO card, analog digital card.
    (a) The light and dark changes of repeated scanning at two positions of a single capillary (repeat scanning 3 times for OCTA at each location). (b) High-speed OCTA image. The white dashed lines indicate the two scanning positions, and the yellow box illustrates the intermittent phenomenon produced by capillaries. The red solid lines and the green dashed lines represent two additional pairs of scanning positions that are equidistant from each other. (c) Different scanning protocols. The fast axis and slow axis waveforms are the signals that trigger the two galvanometers, respectively. (d) Capillary B-scan image processing at two locations. (e) Encoded images showing the variations in brightness over time of the same capillary cross-section at scanning positions S1 and S2.
    (a) High-speed OCTA image. (b)–(g) Local magnified images of the same position at different time points.
    Encoding of light and dark changes in the same capillary at different scanning intervals.
    Correlation of capillary light and dark coding. (a)–(e) Correlation of capillary light and dark coding with equal proportional increase in scanning spacing. (f) Correlation of light and dark coding of scanning positions across bifurcated capillaries.
    Changes in blood flow velocity in the same capillaries in the retina of mouse during anesthesia (n = 5, five measurements are taken at each location). Error bars = SEM.
    • Table 1. Summary of Different OCTA and Alternating Scan Imaging Protocols

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      Table 1. Summary of Different OCTA and Alternating Scan Imaging Protocols

       One B-scan timeRepeat timesB-scan number per second
      High-speed OCTA1 ms40.49 vps
      OCTA B-scan1 ms3166.7 fps
      En-OCTA B-scan1 ms1500 fps
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    Shujiang Chen, Kaixuan Hu, Wei Yi, Fuwang Wu, Yi Wan, Lei Zhang, Jianmei Li, Aiqun Wang, Weiye Song, "Method for measuring retinal capillary blood flow velocity by encoded OCTA," Chin. Opt. Lett. 23, 041701 (2025)

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    Paper Information

    Category: Biophotonics

    Received: Jul. 30, 2024

    Accepted: Nov. 4, 2024

    Posted: Nov. 4, 2024

    Published Online: Apr. 24, 2025

    The Author Email: Weiye Song (songweiye@sdu.edu.cn)

    DOI:10.3788/COL202523.041701

    CSTR:32184.14.COL202523.041701

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