Laser & Optoelectronics Progress, Volume. 61, Issue 6, 0618003(2024)
Lensless Coded Ptychographic Microscopy Imaging: Principle and Recent Progress (Invited)
Fig. 1. Traditional ptychographic imaging technology[12]. (a) System schematic diagram; (b) forward imaging model; (c) iterative phase recovery process
Fig. 2. The number of ptychography-related publications and citations from 2004 to 2023
Fig. 3. Fourier ptychographic microscopy[45]. (a) System schematic diagram; (b) system device; (c)(d) raw images,recovered color images, and recovered phase images of blood smear slide and stained tissue slice
Fig. 5. Imaging model and reconstruction process of coded ptychography[12]. (a) The forward imaging model of coded ptychography; (b) the iterative phase retrieval process
Fig. 6. Schematic diagrams of different coded ptychographic systems[12]. (a) Wavelength-multiplexed coded ptychography; (b) parallel coded ptychography; (c) temporal-similarity constraint coded ptychography; (d) optofluidic coded ptychography; (e) ptychographic whole slide scanner; (f) rotational coded ptychography; (g) synthetic aperture coded ptychography; (h) depth-multiplexed coded ptychography
Fig. 7. Hardware platforms for different coded ptychographic implementations[44, 74-77, 79]. (a) Parallel coded ptychography ;(b) integrated ptychographic sensor for large-scale lensless microbial monitoring with high spatiotemporal resolution; (c) optofluidic ptychography with a microfluidic chip for sample delivery; (d) color-multiplexed ptychographic whole slide scanner; (e) rotational coded ptychography; (f) depth-multiplexed coded ptychography
Fig. 8. Resolution performance using different coded layers[74, 76]. (a1) Thick coded layer coated with microbeads; (b1) single-layer thin coded layer coated with microbeads; (c1) the recovered transmission profile of the monolayer fish blood cells; (d1) the recovered transmission profile of the monolayer goat blood cells; (a2)‒(d2) the recovered images of the resolution target corresponding to different coded layers
Fig. 9. Digital refocusing of coded ptychographic imaging[76]. (a) Large field of view imaging results based on digital refocusing; (b) focus map corresponding to Fig. 9 (a); (c1) (c2) the recovered intensity of the object exit waves of different regions; (d1) (d2) the refocused intensity images of different regions; (e1) (e2) the refocused phase images of different regions
Fig. 10. Slow-varying phase imaging[44, 74]. (a1)‒(c1) Object images corresponding to optical prism, biconvex lens, and bacterial colonies; (a2)‒(c2) the captured raw images of Figs. 10(a1)-(c1);(a3)‒(c3) the recovered wrapped phase images of Figs. 10(a1)-(c1); (a4)‒(c4) the recovered unwrapped phase images of Figs. 10(a1)-(c1)
Fig. 12. Whole slide imaging and digital pathology related applications[44, 76]. (a1) (b1) The focus maps for WSI; (a2) (b2) the virtually stained whole slide images; (a3) (b3) the ground-truth images captured by a 40×, 0.95 NA lens; (a4) (b4) the difference maps; (c1) (c2) the recovered intensity and phase images of a slide labelled with the Ki-67 markers; (d) the segmentation results using the deep neural network; (e1) (e2) zoomed-in views of the highlighted regions in (c2) and (d); (f) the measurement of dry mass and cell area for the Ki-67 positive and negative cells; (g) the histogram analysis of the cell eccentricity, cell area, dry mass, and average phase
Fig. 13. High-throughput cytometric analysis of blood cells and urine sediments[77]. (a1) Recovered whole slide phase image of a blood smear via the rotational coded ptychographic platform, inset shows the locations of WBCs and parasites based on the automatic segmentation and tracking process; (a2) recovered phase of the small region in Fig. 13(a1); (a3) scatter plot of WBCs and parasites based on cell area and average phase; (b1) recovered whole slide phase image of a urine sediment sample via the rotational coded ptychographic platform; (b2) recovered phase images of different elements in the urine sediment slides
Fig. 14. Time-lapse monitoring of bacterial and cell growth[74, 79]. (a) Recovered phase images of E.coli bacterial cells at different time points; (b) quantitative phase analysis results of bacterial colonies; (c1) recovered phase images of 15 bacterial colonies over a centimeter-scale area; (c2) monitoring of bacterial growth process under different antibiotic concentrations; (c3) the variation curve of bacterial dry mass over time; (d) time-lapse monitoring of the HEK 293FT cells via the depth-multiplexed coded ptychographic platform
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Chengfei Guo, Jingyan Li, Shaowei Jiang, Xiaopeng Shao, Guoan Zheng. Lensless Coded Ptychographic Microscopy Imaging: Principle and Recent Progress (Invited)[J]. Laser & Optoelectronics Progress, 2024, 61(6): 0618003
Category: Microscopy
Received: Nov. 29, 2023
Accepted: Dec. 25, 2023
Published Online: Mar. 22, 2024
The Author Email: Chengfei Guo (guochengfei@xidian.edu.cn), Shaowei Jiang (jiangsw@hdu.edu.cn), Xiaopeng Shao (xpshao@xidian.edu.cn)
CSTR:32186.14.LOP232582