Axial gradient excitation accelerates volumetric imaging of two-photon microscopy

Yufeng Gao; Xianyuan Xia; Lina Liu; Ting Wu; Tingai Chen; Jia Yu; Zhili Xu; Liang Wang; Fei Yan; Zhuo Du; Jun Chu; Yang Zhan; Bo Peng; Hui Li; Wei Zheng

doi:10.1364/PRJ.441778

Photonics Research, Volume. 10, Issue 3, 687(2022)

Axial gradient excitation accelerates volumetric imaging of two-photon microscopy

Yufeng Gao^1,2、†, Xianyuan Xia^1,2、†, Lina Liu^1,2, Ting Wu^1,2, Tingai Chen^1,2, Jia Yu^1,2, Zhili Xu³, Liang Wang^1,2, Fei Yan³, Zhuo Du^4,5, Jun Chu^1,2, Yang Zhan⁶, Bo Peng⁷, Hui Li^1,2,8、*, and Wei Zheng^1,2,9、*

Author Affiliations

¹Research Center for Biomedical Optics and Molecular Imaging, Shenzhen Key Laboratory for Molecular Imaging, Guangdong Provincial Key Laboratory of Biomedical Optical Imaging Technology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China

²CAS Key Laboratory of Health Informatics, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China

³Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China

⁴State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China

⁵Key Laboratory of Genetic Network Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China

⁶Brain Cognition and Brain Disease Institute, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China

⁷Centre for Micro Nano Systems and Bionic Medicine, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China

⁸e-mail: hui.li@siat.ac.cn

⁹e-mail: zhengwei@siat.ac.cn

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Supplementary Materials

Visualization 1 | EGFP labeled HEK293 cells were imaged by Grad-TPM and Gauss-TPM with the same volume size and acquisition speed. The photobleaching of Grad-TPM imaging is found to be obviously lower than that of the Gauss-TPM imaging and thus more favorable for obse

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Visualization 2 | HepG2 cells with DyLight 488 labeled membrane and PI labeled nucleus were imaged by Grad-TPM and Gauss-TPM with the same volume size and acquisition speed. PI can’t cross the membrane of live cells and appear bright only if it binds to the nucleic ac

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Visualization 3 | Macrophages are phagocytizing fluorescent beads wandering around. The time is shown at the lower left corner as min:s.

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Yufeng Gao, Xianyuan Xia, Lina Liu, Ting Wu, Tingai Chen, Jia Yu, Zhili Xu, Liang Wang, Fei Yan, Zhuo Du, Jun Chu, Yang Zhan, Bo Peng, Hui Li, Wei Zheng, "Axial gradient excitation accelerates volumetric imaging of two-photon microscopy," Photonics Res. 10, 687 (2022)

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Paper Information

Category: Imaging Systems, Microscopy, and Displays

Received: Sep. 28, 2021

Accepted: Jan. 10, 2022

Published Online: Feb. 22, 2022

The Author Email: Hui Li (hui.li@siat.ac.cn), Wei Zheng (zhengwei@siat.ac.cn)

DOI:10.1364/PRJ.441778

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