In order to estimate the position and orientation of an object with a single camera, a novel measurement method based on pinhole camera model with five reference points is presented. Taking the specially designed planar target with the monocular vision system, the projection line of the reference points is built. According to the projection model, the coordinates of the reference points in the camera coordinate system are estimated with the least-squares algorithm. Thus the position and orientation of the target are worked out. Experimental result shows that the measurement precision of angle is less than 0.2°, and that of displacement is less than 0.1 mm.