Fig. 1. Schematic diagram of cryo-CLEM. (a) Cell culturing; (b) fluorescent labeling; (c) fast freezing; (d) fluorescent imaging; (e) FIB milling; (f) cryo-ET imaging

Fig. 2. Commercial plunge freezers and high pressure freezers. (a) VitrobotTM from FEI; (b) CP3 from Gatan; (c) EMPact from Leica; (d) HPM from Bal-Tec

Fig. 3. Commercial cryo stages and prototypes. (a) CLM77K from Instec; (b) CMS196 from Linkam; (c) CorrSight from FEI; (d) Cryo stage proposed by Li^[19]; (e) Cryo stage proposed by Xu^[20]

Fig. 4. (a) Schematic diagram and (b) flow chart of imaging process of independent cryo-CLEM system

Fig. 5. Schematic diagrams of cryo supper resolution fluorescent microscopy. (a) cryo-STED; (b) cryo-SMLM; (c) cryo-SIM; (d) cryo-Airyscan

Fig. 6. (a) Schematic diagram and (b) block diagram of imaging process of integrated cryo-CLEM system