Acta Optica Sinica, Volume. 45, Issue 6, 0600001(2025)

Research Progress and Prospects of Optical Technologies for Detecting Bioaerosols (Invited)

Zhongwei Huang1,2、*, Yuanzong Ji1, Yongkai Wang1, Tian Zhou1, Wuren Li2, and Xuefei Huo1
Author Affiliations
  • 1Key Laboratory for Semi-Arid Climate Change of the Ministry of Education, College of Atmospheric Sciences, Lanzhou University, Lanzhou 730000, Gansu , China
  • 2Collaborative Innovation Center for Western Ecological Safety, Lanzhou University, Lanzhou 730000, Gansu , China
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    Figures & Tables(16)
    Bioaerosol optical detection technologies and equipment widely used by scholars over world
    Schematic diagram of the basic principle of laser induced fluorescence technology
    Fluorescence microscopes[23]. (a) Schematic diagram of transmission fluorescence microscope strcuture; (b) schematic diagram of fall-light fluorescence microscope structure
    Schematic diagram of multi-wavelength Raman-fluorescence lidar developed by researchers from Lanzhou University[31]
    Schematic diagram of working principle of flow cytometer[37]
    Schematic diagram of detection principle of ultraviolet aerodynamic particle size spectrometer (UV-APS)[39]
    Detection principle and data classification method of WIBS. (a) Schematic diagram of WIBS structure[12]; (b) schematic diagram of WIBS data result classification[44]
    Schematic diagram of working principle of Rapid-E+ bioaerosol sensor
    Schematic diagram of detection principle of micro confocal Raman spectrometer[60]
    Main detection principle of SERS[67]
    Main detection principle of CARS[67][CARS photons are generated after pump pulse (1), Stokes pump pulse (2), and probe pulse (3) interact with sample (4)]
    Schematic diagram of detection principle of BAMS[76]
    Schematic diagram of basic principle of biological ATP autofluorescence reactions[12]
    ATP bioluminescence detection of bioaerosols. (a) ATP-based bioluminescence assay for real-time detection of bioaerosols[84]; (b) ATP-based bioluminescence assay on microfluidic chips for bacterial bioaerosols[85]
    Schematic diagram of basic detection principle of LIBS[87]
    • Table 1. Detection principles, advantages, and disadvantages of different optical technologies for detecting bioaerosols

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      Table 1. Detection principles, advantages, and disadvantages of different optical technologies for detecting bioaerosols

      TechnologyDetection principleAdvantageDisadvantage
      Fluorescence scatteringUses laser to excite biological molecules, causing specific fluorescence signalsHighly sensitive; real-time detection for various biological moleculesRequire specific equipment; background noise can affect fluorescence signals
      Raman scatteringLaser irradiation generates Raman scattering signalsProvides molecular structure information, no need for sample labelingWeak signal; need sensitive detectors and complex data processing
      Mass spectrometryIonize sample to analyze its molecular compositionHigh sensitivity and specificity; capable of quantitative analysisCostly equipment; need ionization and vacuum systems
      Biological ATP AutofluorescenceDetect natural fluorescence of ATP in cellsRapid detection; no complex sample preparation neededRelatively lower sensitivity; limited applicability.
      LIBSUse high-energy laser to create plasma, and analyze its light to identify elementsRapid detection; capable of detecting multiple elementsNeed high-energy laser for low organic molecule sensitivity
      Microfluidic chipUse micro-scale channels and zones for sample processing and detectionLow sample requirement; capable of high-throughput and integrated operationsNeed complex design, fabrication, and special detection tools
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    Zhongwei Huang, Yuanzong Ji, Yongkai Wang, Tian Zhou, Wuren Li, Xuefei Huo. Research Progress and Prospects of Optical Technologies for Detecting Bioaerosols (Invited)[J]. Acta Optica Sinica, 2025, 45(6): 0600001

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    Paper Information

    Category: Reviews

    Received: Aug. 20, 2024

    Accepted: Sep. 21, 2024

    Published Online: Mar. 4, 2025

    The Author Email: Zhongwei Huang (huangzhongwei@lzu.edu.cn)

    DOI:10.3788/AOS241448

    CSTR:32393.14.AOS241448

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