Fig. 1. Two-photon DH-PSF image at different depth z, and linear relationship between rotation angle θ and z. (a) Two-photon DH-PSF image at different depth z; (b) linear relationship between rotation angle θ and z

Fig. 2. Schematic diagram of DH-MTPLSM system

Fig. 3. CGH phase pattern for two-layer multifocal array, conventional two-photon excitation fluorescence image of multifocal array in solution of eosin dye, and DH-PSF image of same array. (a) CGH phase pattern for two-layer multifocal array; (b) conventional two-photon excitation fluorescence image of multifocal array in solution of eosin dye; (c) DH-PSF image of same array

Fig. 4. Data process for DH-MTPLSM. (a) Original images; (b) filtering with a pair of Gaussian pinholes; (c) image reconstruction process

Fig. 5. Images of fluorescent beads with diameter of 100 nm. (a) Wide field image; (b) DH-MTPLSM reconstruction image; (c) yz projection of wide field image; (d) yz projection of DH-MTPLSM reconstruction image; (e)-(g) intensity profiles in x, y, and z directions, respectively, and their Gaussian fitting curves

Fig. 6. Imaging results of mouse kidney section. (a) WF image at focal plane; (b) reconstructed images at different axial depths