In order to screen high-yielding, stable, high-quality, stress-resistant, and early-fruiting Morchella spp. germplasm resources suitable for cultivation and promotion in Wuling Mountain area, 13 key agronomic traits of 18 Morchella spp. varieties were statistically analyzed. Two comprehensive evaluation methods, the Grey Relational Analysis (GRA)and the Distance to the Ideal Solution method based on Technique for Order Preference by Similarity to an Ideal Solution (DTOPSIS)were combined to compare and analyze the differences among the varieties, aiming to provide a new comprehensive evaluation approach for the selection and breeding of new Morchella spp. varieties. The results indicated that under equal weight settings, the evaluation outcomes of the GRA and DTOPSIS methods were different. The GRA results showed that the maximum difference rate of the weighted grey relational coefficient (R_i')between tested varieties and the ideal variety was 41. 64%, while the difference rate of the equally weighted coefficient(R_i)was 34. 73%. Since R_i' was greater than R_i, using the weighted coefficient(R_i')for variety ranking proved more reasonable. The DTOPSIS results revealed that the maximum difference rate in relative closeness(C_i) between tested varieties and the ideal variety was 68. 30%. As this difference rate was greater than that of R_i', DTOPSIS was better able to reflect differences and merits among Morchella spp. varieties, making it more suitable for comprehensive evaluation of Morchella spp. germplasm. Based on the comprehensive evaluation using the DTOPSIS method, the varieties No. 19, No. 1, No. 18, No. 15 of Morchella eximia, and Morchella sextelata No. 21 were found to be suitable for cultivation in the Wuling Mountain area. These varieties can be further tested, demonstrated and promoted, thereby laying a foundation for the selection and breeding of Morchella spp. in the Wuling Mountain area.