Self-supervised denoising for enhanced volumetric reconstruction and signal interpretation in two-photon microscopy

Jie Li; Liangpeng Wei; Xin Zhao

doi:10.1364/PRJ.563812

Photonics Research, Volume. 13, Issue 8, 2418(2025)

Self-supervised denoising for enhanced volumetric reconstruction and signal interpretation in two-photon microscopy

Jie Li^1,2, Liangpeng Wei³, and Xin Zhao^1,2、*

¹National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, Engineering Research Center of Trusted Behavior Intelligence, Ministry of Education, Tianjin Key Laboratory of Intelligent Robotic (tikLIR), Institute of Robotics & Automatic Information System (IRAIS), Nankai University, Tianjin 300350, China

²Shenzhen Research Institute of Nankai University, Shenzhen 518083, China

³School of Biomedical Engineering and Technology, Tianjin Medical University, Tianjin 300070, China

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Supplementary Materials

Visualization 1 | Supplementary Visualization 1—Simulated volumetric structural imaging of neurons.

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Visualization 2 | Supplementary Visualization 2—Two-photon volumetric imaging of single neurons in mouse cortex.

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Visualization 3 | Supplementary Visualization 3—Two-photon volumetric imaging of neuronal population in mouse cortex.

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Visualization 4 | Supplementary Visualization 4—Two-photon volumetric imaging of neuronal dendrites of mouse.

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Visualization 5 | Supplementary Visualization 5—Confocal microscopy volumetric structural imaging of penicillin.

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Visualization 6 | Supplementary Visualization 6—Two-photon volumetric imaging of cerebrovasculature of mouse.

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Visualization 7 | Supplementary Visualization 7—Expansion microscopy volumetric structural imaging of the intestine of mouse embryos.

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Visualization 8 | Supplementary Visualization 8—Computed tomography of human thoracoabdominal body.

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Visualization 9 | Supplementary Visualization 9—Scanning electron microscopy volumetric imaging of cells from mouse cortex.

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Jie Li, Liangpeng Wei, Xin Zhao, "Self-supervised denoising for enhanced volumetric reconstruction and signal interpretation in two-photon microscopy," Photonics Res. 13, 2418 (2025)

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Paper Information

Category: Image Processing and Image Analysis

Received: Mar. 31, 2025

Accepted: May. 22, 2025

Published Online: Aug. 4, 2025

The Author Email: Xin Zhao (zhaoxin@nankai.edu.cn)

DOI:10.1364/PRJ.563812

CSTR:32188.14.PRJ.563812

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