Concentration Quantification Method Based on Single-Wavelength Excitation via Grüneisen-Relaxation Nonlinear Photoacoustic Effect

Xiatian Wang; Lü Shengmiao; Zhihua Xie; Jingqin Chen; Xiaojing Gong; Liquan Dong

doi:10.3788/AOS231317

Acta Optica Sinica, Volume. 43, Issue 23, 2317001(2023)

Concentration Quantification Method Based on Single-Wavelength Excitation via Grüneisen-Relaxation Nonlinear Photoacoustic Effect

Xiatian Wang^1,2, Lü Shengmiao², Zhihua Xie², Jingqin Chen², Xiaojing Gong^2、**, and Liquan Dong^1,3、*

Author Affiliations

¹Beijing Key Laboratory for Precision Optoelectronic Measurement Instrument and Technology, School of Optics and Photonics, Beijing Institute of Technology, Beijing 100081, China

²Research Center for Biomedical Optics and Molecular Imaging, Shenzhen Key Laboratory for Molecular Imaging, Guangdong Provincial Key Laboratory of Biomedical Optical Imaging Technology, CAS Key Laboratory of Health Informatics, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, Guangdong , China

³Yangtze Delta Region Academy of Beijing Institute of Technology (Jiaxing), Jiaxing 314019, Zhejiang , China

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Figures & Tables(9)

Fig. 1. Error of concentration quantification results. (a) Triangle of error coefficients for linear and nonlinear methods; (b) concentration quantification error of two methods

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Fig. 2. Schematic of experiment system

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Fig. 3. Scatter diagrams of mass concentration-amplitude of red dye. (a) Linear signals; (b) nonlinear signals

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Fig. 4. Experimental results of red dye. (a) Results of concentration quantification; (b) relative error (absolute value)

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Fig. 5. Scatter diagrams of mass concentration-signal amplitude of Rhodamine 6G. (a) Linear signal; (b) nonlinear signal

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Fig. 6. Experimental results of Rhodamine 6G. (a) Results of concentration quantification; (b) relative error (absolute value)

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Table 1. Simulation results of concentration quantification
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Table 1. Simulation results of concentration quantification
R_SB
c_m /
（mg·mL^-1）
c_Lm /
（mg·mL^-1）
c_Nm /
（mg·mL^-1）
1.00 1.00 2.00 1.41
2.00 2.00 3.00 2.24
3.00 3.00 4.00 3.16
4.00 4.00 5.00 4.12
5.00 5.00 6.00 5.10

Table 2. Signal amplitudes of red dye solution and blue dye solution
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Table 2. Signal amplitudes of red dye solution and blue dye solution
Sample Concentration /（mg·mL^-1） Normalized amplitude of signal R_{SB_red}
Linear Nonlinear
Red dye 2 0.20 0.02 1.11
4 0.42 0.20 2.33
6 0.64 0.42 3.56
8 0.83 0.69 4.61
10 1.00 1.00 5.56
Blue dye 6 0.18 0.04 —

Table 3. Signal amplitudes of Rhodamine 6G solution and hemoglobin solution
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Table 3. Signal amplitudes of Rhodamine 6G solution and hemoglobin solution
Sample Concentration /（mg·mL^-1） Normalized amplitude of signal R_{SB_Rho6G}
Linear Nonlinear
Rhodamine 6G 1 0.15 0.02 1.00
2 0.23 0.13 1.53
4 0.48 0.35 3.20
6 0.76 0.62 5.07
8 1.00 1.00 6.67
Hemoglobin 150 0.15 0.02 —

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Xiatian Wang, Lü Shengmiao, Zhihua Xie, Jingqin Chen, Xiaojing Gong, Liquan Dong. Concentration Quantification Method Based on Single-Wavelength Excitation via Grüneisen-Relaxation Nonlinear Photoacoustic Effect[J]. Acta Optica Sinica, 2023, 43(23): 2317001

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