Fig. 1. Beam path diagram and mechanical drawing of the compact LSFM

Fig. 2. Schematic diagram of the 3D imaging process and the 3D reconstruction of the data in LSFM

Fig. 3. Flowchart of the MSSR algorithm

Fig. 4. Simulations of MSSR algorithm distinguishing two point sources spaced at Rayleigh or Sparrow criterion

Fig. 5. Test of the system's lateral resolution with 100nm diameter fluorescence beads

Fig. 6. Test of the system's lateral resolution with the Argolight commercial FM resolution chart

Fig. 7. Result comparison between LSFM direct imaging and MSSR⁰ processing for Spinach root slice

Fig. 8. The process of field of view stitching and 3D reconstruction of Spinach root slice

Fig. 9. Bicolor imaging result of mouse intestine slice（Green channel，Blood Vessels；Red Channel，Nuclei）