Fig. 1. OCT system diagram

Fig. 2. Fish body orientation fixed images

Fig. 3. Imaging of zebrafish in 24‒72 hpf. (a) Micrograph of zebrafish; (b) OCT 3D stereogram; (c)‒(e) surface to the inside of the chromatogram obtained by OCT scan along X-Y direction

Fig. 4. Imaging of zebrafish in 96 hpf (Ⅰ) and 168 hpf (Ⅱ). (a) Micrograph of zebrafish; (b) OCT 3D stereogram; (c) (d) surface to the inside of the chromatogram obtained by OCT scan along X-Y direction

Fig. 5. Imaging of zebrafish in 15 dpf (Ⅰ) and 45 dpf (Ⅱ). (a) Micrograph of zebrafish; (b) OCT 3D stereogram; (c)(d) surface to the inside of the chromatogram obtained by OCT scan along the X-Y direction

Fig. 6. Imaging of zebrafish female (Ⅰ) and male (Ⅱ) in 105 dpf. (a) Micrograph of zebrafish; (b) OCT 3D stereogram; (c)(d) surface to the inside of the chromatogram obtained by OCT scan along the X-Y direction

Fig. 7. Changes in the zebrafish brain. (a) X-Y OCT scan of zebrafish brain during its growth stage, the dashed box represents the outline of the brain; (b) brain area changes of zebrafish

Fig. 8. Eye changes in zebrafish. (a) X-Z OCT scan of zebrafish eyes during growth stage; (b) eye axis length variation of zebrafish; (c) retinal thickness variation of zebrafish

Fig. 9. X-Y OCT scan of zebrafish heart during growth stage

Fig. 10. Extracted heart rate of zebrafish. (a) M-Mode diagram of embryonic zebrafish; (b) pulse amplitude of embryonic zebrafish; (c) M-Mode diagram of zebrafish in adult stage; (d) pulse amplitude of zebrafish in adult stage

Fig. 11. Quantization of cardiac parameters. (a) Heart rate variations of zebrafish; (b) ventricular area variations of zebrafish