Growth and development of zebrafish are valuable areas of research as these fish serve as an excellent animal model in biomedicine. This study used optical coherence tomography (OCT) to continuously monitor the three-dimensional imaging of zebrafish development, creating a comprehensive model for their growth and development. Furthermore, the study evaluated the morphology of key organs, such as the eyes, brain, and heart, through quantitative analysis. The findings revealed that zebrafish undergo transition from inner to outer ovary development primarily during the embryonic phase, with the most significant changes in shape and internal composition occurring during the larval phase. Basic organ development is completed during the juvenile stage, whereas the gonads reach maturity during the adult stage. Although the brain does not reach full maturity until adulthood, eye development is primarily functional during the embryonic stage. This growth pattern is significantly different from that observed in other species. Moreover, during the developmental stage of zebrafish, there is an approximately 60-fold increase in the ventricular area. This study successfully captured in vivo images of the entire zebrafish developmental process and examined the growth status of its internal organs. These results provide crucial information for future studies on growth and development using zebrafish as a model.