Femtosecond fiber laser at 780 nm for two-photon autofluorescence imaging

Wan Yang; Danlei Wu; Runlong Wu; Guanyu Liu; Bingying Chen; Lishuang Feng; Zhigang Zhang; Aimin Wang

doi:10.3788/COL201917.071405

Chinese Optics Letters, Volume. 17, Issue 7, 071405(2019)

Femtosecond fiber laser at 780 nm for two-photon autofluorescence imaging

Wan Yang¹, Danlei Wu^1,2, Runlong Wu¹, Guanyu Liu¹, Bingying Chen¹, Lishuang Feng², Zhigang Zhang¹, and Aimin Wang^1、*

¹State Key Laboratory of Advanced Optical Communication System and Networks, School of Electronics Engineering and Computer Science, Peking University, Beijing 100871, China

²Key Laboratory of Precision Opto-Mechatronics Technology, Ministry of Education, School of Instrumentation Science & Optoelectronics Engineering, Beihang University, Beijing 100191, China

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Fig. 1. Schematic diagram of the laser system. PBS, polarization beam splitter; SM LD, single-mode laser diode; MM LD, multi-mode laser diode; WDM, wavelength division multiplexer; ISO, isolator.

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Fig. 2. Results of the Er:fiber laser. (a) Calculated nonlinear phase shift and residual TOD in the FCPA system, (b) spectra and intensity autocorrelation traces under different DCF lengths.

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Fig. 3. Experimental results of the frequency doubling. (a) RF spectrum of the oscillator, (b) output average power, (c) optical spectrum, (d) intensity autocorrelation trace of the frequency doubled pulse.

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Fig. 4. Optical spectra of frequency doubling under different DCF lengths.

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Fig. 5. (a) Experimental result of TPAM imaging of the rabbit intestine tissue ex vivo. Image size: 248 μm. Scale bar: 20 μm. (b) Experimental result of TPAM imaging of the human skin in vivo at different depths. Image size: 130 μm. Scale bar: 20 μm.

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Table 1. Dispersion of the FCPA System
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Table 1. Dispersion of the FCPA System
Component Other Fibers Fiber Stretcher Grating Compressor
Type SMF-28 DCF 966 l/mm 600 l/mm
$β_{2}$ ( ${fs}^{2} / mm$ ) $- 22$ 170 $- 14871$ $- 3107$
$β_{3}$ ( ${fs}^{3} / mm$ ) 127 $- 983$ 137369 12085
$β_{3} / β_{2}$ (fs) $- 5.77$ $- 5.78$ $- 9.24$ $- 3.89$

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Wan Yang, Danlei Wu, Runlong Wu, Guanyu Liu, Bingying Chen, Lishuang Feng, Zhigang Zhang, Aimin Wang, "Femtosecond fiber laser at 780 nm for two-photon autofluorescence imaging," Chin. Opt. Lett. 17, 071405 (2019)

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