Real-Time Tracking Focal Plane Method of Digital Slide Scanner

Fig. 4. Tilt plane image and Lorenz fitting curve of sharpness-pixel scatter. (a) Tilt plane image; (b) Lorenz fitting curve of sharpness-pixel scatter

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Fig. 5. Defocus distance-pixel scatter and linear fitting curve

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Fig. 6. Experimental setup for digital slide scanner based on tilt camera

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Fig. 7. Cases of successful and failure focusing. (a) Case of successful focusing; (b) case of failure focusing

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Fig. 8. Flow chart of the scanning and real-time focusing

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Fig. 9. Digital slide comparison of two kinds of methods. (a) Digital slide based on traditional modeling method; (b) digital slide based on real-time dynamic focusing method

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Table 1. Experimental data of focusing error

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Table 1. Experimental data of focusing error

$Δ Z_{m}$	$Δ Z_{c}$	$Δ Z_{e}$	$Δ Z_{m}$	$Δ Z_{c}$	$Δ Z_{e}$
13.05	12.31	0.74	-1.15	-0.83	-0.37
12.15	11.5	0.65	-2.05	-1.77	-0.28
11.05	10.4	0.65	-2.95	-2.58	-0.37
10.05	9.51	0.54	-4.05	-3.78	-0.27
9.05	8.42	0.63	-5.15	-5.03	-0.12
7.95	7.64	0.31	-6.05	-5.91	-0.14
7.05	6.61	0.44	-7.05	-6.58	-0.47
6.05	5.59	0.46	-8.15	-7.78	-0.37
5.10	4.75	0.35	-9.05	-8.52	-0.53
4.15	3.94	0.21	-10.05	-9.43	-0.62
3.05	2.86	0.19	-11.15	-10.62	-0.53
2.05	1.67	0.38	-12.05	-11.41	-0.64
1.15	1.02	0.13	-13.10	-12.25	-0.85
0.05	0.12	-0.07