Fig. 1. Morphology of the oocyte cultured in vitro for 12 h under the action of different concentrations of SOV at the periods of (a) GV, (b) GVBD, (c) PB and (d) parthenogenetic activation; (e) statistical data in different meiosis processes

Fig. 2. Fluorescence co-localization of dynein, α-tubulin and chromosome of the oocyte cultured in vitro for 12 h at different meiosis stages. (a) Normal oocyte; (b) partial enlargement of the white square part of MII oocyte andthe abnormal oocyte

Fig. 3. Fluorescence co-localization of α-tubulin of the oocyte cultured in vitro for 12 h at different meiosis stages. (a) Normal oocyte with normal spindle; (b)-(h) abnormal oocytes with various spindles; (i) statistic data of abnormal MI-MII spindle structures

Fig. 4. (a) Normal chromosome structure; (b)-(d) abnormal chromosome structures; (e) statistic data of abnormal MI-MII chromosome structures

Fig. 5. Images of oocyte spindle obtained by (a) fluorescence microscope and (b) STORM at the same position; (c) fluorescence intensity of the spindle fibers pointed by arrows; (d) fluorescence intensity of the spindle fibers pointed by straight lines

Fig. 6. Reconstructed images of the normal oocyte spindle obtained by (a) 2D STORM and (b) 3D STORM; reconstructed images of the abnormal oocyte spindle obtained by (c) 2D STORM and (d) 3D STORM