Combining the discipline advantages and resources of university laboratories, a series of organic optoelectronics comprehensive experimental courses have been constructed with the problem-based learning (PBL) model. With diversified teaching content and methods, the ways of cultivation talents with keen problem awareness, broad scientific perspective, flexible thinking ability and team collaboration ability have been explored. These provide theoretical support and practical guidance for the construction of comprehensive open innovation experimental courses in university laboratories, and is of great significance for promoting the improvement of higher education quality and the innovation of talent training ways.