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Acta Optica Sinica, Volume. 39, Issue 7, 0718001(2019)

Improvement of Imaging Resolution of Fluorescence Emission Difference Super-Resolution Microscopy with Annular Pupil

Suhui Deng1,2, Jie Hu1, Yiping Xiao1, and Mingping Liu1、*
Author Affiliations
  • 1 Information Engineering School of Nanchang University, Nanchang, Jiangxi 330031, China
  • 2 Division of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China
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    Schematic of light path

    Fig. 1. Schematic of light path

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    Normalized PSF distributions of hollow focal spots

    Fig. 2. Normalized PSF distributions of hollow focal spots

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    Normalized PSF distributions of extended solid spot (dashed curve) and hollow focal spot with reduced size by using annular pupil (solid curve) under confocal mode

    Fig. 3. Normalized PSF distributions of extended solid spot (dashed curve) and hollow focal spot with reduced size by using annular pupil (solid curve) under confocal mode

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    Normalized PSF distributions under different imaging modes. (a) Conventional confocal imaging; (b) dfFED imaging without pupil filter; (c) dfFED imaging with annular pupil filter; (d) corresponding intensity profiles of (a), (b), and (c) along central axis

    Fig. 4. Normalized PSF distributions under different imaging modes. (a) Conventional confocal imaging; (b) dfFED imaging without pupil filter; (c) dfFED imaging with annular pupil filter; (d) corresponding intensity profiles of (a), (b), and (c) along central axis

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    Simulated results of sample consisting of six points. (a) Sample pattern; (b) dfFED imaging without pupil; (c) dfFED imaging with annular pupil; (d) normalized intensity profiles along dashed lines shown in (b) and (c)

    Fig. 5. Simulated results of sample consisting of six points. (a) Sample pattern; (b) dfFED imaging without pupil; (c) dfFED imaging with annular pupil; (d) normalized intensity profiles along dashed lines shown in (b) and (c)

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    Simulated results of sample consisting of six lines. (a) Sample pattern; (b) dfFED imaging without pupil; (c) dfFED imaging with annular pupil; (d) normalized intensity profiles along dashed lines shown in (b) and (c)

    Fig. 6. Simulated results of sample consisting of six lines. (a) Sample pattern; (b) dfFED imaging without pupil; (c) dfFED imaging with annular pupil; (d) normalized intensity profiles along dashed lines shown in (b) and (c)

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    Simulated results of spoke-like sample. (a) Sample pattern; (b) dfFED imaging without pupil; (c) dfFED imaging with annular pupil; (d) normalized intensity profiles along dashed lines shown in (b) and (c)

    Fig. 7. Simulated results of spoke-like sample. (a) Sample pattern; (b) dfFED imaging without pupil; (c) dfFED imaging with annular pupil; (d) normalized intensity profiles along dashed lines shown in (b) and (c)

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    Suhui Deng, Jie Hu, Yiping Xiao, Mingping Liu. Improvement of Imaging Resolution of Fluorescence Emission Difference Super-Resolution Microscopy with Annular Pupil[J]. Acta Optica Sinica, 2019, 39(7): 0718001

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    Paper Information

    Category: Microscopy

    Received: Jan. 23, 2019

    Accepted: Mar. 21, 2019

    Published Online: Jul. 16, 2019

    The Author Email: Liu Mingping (mpliu@ncu.edu.cn)

    DOI:10.3788/AOS201939.0718001

    Topics

    laser devices and laser physics
    Lasers and Laser Optics
    Laser physics
    laser manufacturing
    Instrumentation, Measurement and Metrology