The effects of different inoculation periods and mycelial culture conditions on the completion rate of Lentinula edodes artificial bed-log were analyzed. The number of weed mould in the inoculation room and strong cold room was measured after inoculation. Cultivate the inoculated artificial bed-log at 24℃ for 0,4,8, 12, 16, and 20 days respectively, and then transfer them to a mushroom shed for room-temperature cultivation. Compare the effects of temperature-controlled cultivation duration on the completion rate of L. edodes artificial bed-log and the yield of fruit bodies. The results showed that the inoculation periods had no significant effect on the completion rate of L. edodes artificial bed-log, but the mycelial culture conditions had a significant effect on the completion rate of L. edodes artificial bed-log. In comparison, there are more colonies in the strong cold room, indicating that it is a key factor restricting the inoculation environment. Measures such as planning operating routes and controlling personnel movement can be taken to improve the cleanliness of the environment. Temperature-controlled cultivation can improve the completion rate of L. edodes artificial bed-log and the yield of fruit bodies. The optimal condition is to cultivate the L. edodes artificial bed-log for 16 days at a controlled temperature, and then transfer the artificial bed-log to the mushroom shed for room-temperature cultivation. At this conditions, the completion rate of L. edodes artificial bed-logs and the yield of fruit bodies were the highest.