Chinese Optics Letters, Volume. 22, Issue 2, 021101(2024)

High-resolution portable lens-free on-chip microscopy with RGB LED via pinhole array

Qinhua Wang1, Jianshe Ma1, Liangcai Cao2, and Ping Su1、*
Author Affiliations
  • 1Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China
  • 2Department of Precision Instrument, Tsinghua University, Beijing 100084, China
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    Figures & Tables(5)
    (a) The illumination schematic of the RGB LED filtered by a pinhole. (b) The photograph of the RGB LED. (c) The spots generated by three-color beams on the sample plane. (d) The enlarged region corresponding to the white boxed area in (c).
    (a) The illumination schematic of the RGB LED filtered by a pinhole and a diffuser. (b) The red spots obtained when the diffuser is 120 grit (b1), 220 grit (b2), and 600 grit (b3), respectively, and the yellow dashed boxes are the region illuminated by the red beam. (c) The three-color spots obtained when the diffuser is 120 grit (c1), 220 grit (c2), and 600 grit (c3), and the white dashed boxes are the region co-illuminated by the three-color beams.
    (a) The illumination schematic of the RGB LED filtered by a pinhole array. (b) The schematic diagram of the pinhole array. (c) The effect of the pitch of pinholes (Δd) on the size of the region co-illuminated by the three-color beams, and the red region is the valid pitch of the pinholes. (d) The spot of the RGB LED on the sample plane with the pinhole array.
    (a) Schematic diagram of the LFOCM-RGB. (b) Three-color spots on the CMOS obtained with pinhole array. Raw hologram captured at the red light without the pinhole array (c1) and with the pinhole array (c2), and their amplitude distributions reconstructed by multi-wavelength phase retrieval in (c2) and (d2). (c3), (d3) The enlarged regions corresponding to the red boxed areas in (c2), (d2) and the amplitude values along the yellow and red lines.
    Experimental result of pine stem. (a) The raw hologram captured at the red light without the pinhole array and with the pinhole array. (b) The amplitude distributions reconstructed by multi-wavelength phase retrieval corresponding to Area 1, Area 2, and Area 3 without the pinhole array and with the pinhole array.
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    Qinhua Wang, Jianshe Ma, Liangcai Cao, Ping Su, "High-resolution portable lens-free on-chip microscopy with RGB LED via pinhole array," Chin. Opt. Lett. 22, 021101 (2024)

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    Paper Information

    Category: Imaging Systems and Image Processing

    Received: Feb. 7, 2023

    Accepted: Sep. 20, 2023

    Posted: Sep. 21, 2023

    Published Online: Feb. 21, 2024

    The Author Email: Ping Su (su.ping@sz.tsinghua.edu.cn)

    DOI:10.3788/COL202422.021101

    CSTR:32184.14.COL202422.021101

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