Spectroscopy and Spectral Analysis, Volume. 40, Issue 12, 3755(2020)

Mechanism of Isoquercitrin Inhibiting Advanced Glycation Products Formation Based on Fluorescence Spectroscopy Technique

Lu ZHANG1,1, Liang XU1,1, Zong-cai TU1,1, Qi-ming ZHOU1,1, and Wen-na ZHOU1,1
Author Affiliations
  • 1[in Chinese]
  • 11. College of Life Science, National R&D Center for Freshwater Fish Processing, and Engineering Research Center of Freshwater Fish High-value Utilization of Jiangxi Province, Jiangxi Normal University, Nanchang 330022, China
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    Figures & Tables(6)
    Inhibitory activity of isoquercetin on the formation of AGEs on α-La-fructose system
    Influence of isoquercetin on the intrinsic fluorescence spectra of glycated α-La
    Synchronous fluorescence spectra of α-La with different concentrations of isoquercetin with Δλ fixed at 15 nm (a) and 60 nm (b), respectively
    Three-dimensional fluorescence spectra of α-La without (a) and with (b) isoquercetin
    (a) Influence of isoquercetin on the fluorescence emission spectra of α-La; (b) The Stern-Volmer plots of F0/F versus isoquercetin concentrations [Q]; (c) The double logarithmic plots of log[(F0-F)/F] versus log[Q]; (d) The linear plot of lnKa versus 1/T
    • Table 1. The fluorescence quenching constants, binding sites, binding constant and thermodynamic parameters for the interaction of isoquercetin with α-La

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      Table 1. The fluorescence quenching constants, binding sites, binding constant and thermodynamic parameters for the interaction of isoquercetin with α-La

      T/KKSV/(104
      L·mol-1)
      Kq/(1012
      L·mol-1·s-1)
      nKa/(106
      L·mol-1)
      ΔHo/
      (kJ·mol-1)
      ΔSo/
      (kJ·mol-1·K-1)
      RΔGo/
      (kJ·mol-1)
      2986.076.071.299264.70-48.11
      3045.335.331.4727.25-458.79-1.390.999 9-39.84
      3105.145.141.1280.21-31.57
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    Lu ZHANG, Liang XU, Zong-cai TU, Qi-ming ZHOU, Wen-na ZHOU. Mechanism of Isoquercitrin Inhibiting Advanced Glycation Products Formation Based on Fluorescence Spectroscopy Technique[J]. Spectroscopy and Spectral Analysis, 2020, 40(12): 3755

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    Paper Information

    Category: Research Articles

    Received: Nov. 1, 2019

    Accepted: --

    Published Online: Jun. 18, 2021

    The Author Email:

    DOI:10.3964/j.issn.1000-0593(2020)12-3755-06

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