Fig. 1. Schematic diagram of the three-dimensional propagation method of a beam in heterogeneous scattering media

Fig. 2. Light-sheet microscopy imaging simulation architecture. (a) Schematic diagrams of the imaging modes of four light sheet microscopes; (b) cross-sectional view of the illumination beam of four light-sheet microscopes

Fig. 3. Effect of PSF on four light-sheet microscopes with different optical lengths. (a) (b) The PSF at the center of the light sheet field of view and 25 μm away along the z-axis, respectively; (c) (d) the axial FWHM curves as a function of optical length at these positions

Fig. 4. Effect of contrast of images imaged by four light-sheet microscopes with different optical lengths. (a) Cross-sectional view of the fluorescence microsphere model imaging simulation results; (b) (d) magnified views of the white square regions at the center of the field of view and 25 μm along the z-axis from the center in Fig. 4 (a); (c) (e) contrast vs. optical path length curves calculated from the yellow square regions at the center of the field of view and 25 μm along the z-axis from the center in Fig. 4 (a)

Fig. 5. The imaging of the four types of light-sheet microscopy affected by the illumination optical path and the detection optical path, respectively. (a) Simulated imaging cross-sections of different illumination optical lengths; (b) simulated imaging cross-sections of different detection optical lengths; (c) the normalized contrast degradation curves at different optical lengths at the illumination and detection optical paths,calculated for square regions at the center of the field of view and 25 μm along the z-axis from the center