Chinese Journal of Lasers, Volume. 45, Issue 5, 507001(2018)
Remote Raman Spectra Detection of Human Skin Fibroblasts
Figures & Tables(7)
Fig. 1. (a) Microphotograph of human skin fibroblasts; (b) microphotograph of mixed culture of human skin fibroblasts with the tapered SERS fiber probe and gold sol
Fig. 2. SEM images of human skin fibroblasts grown on the gold nanoparticles modified tapered fiber proble
Fig. 3. Schematics of experimental setups. (a) SERS tapered fiber probe remote detection; (b) direct detection of human skin fibroblasts SERS spectra
Fig. 4. (a) Raman spectra of human skin fibroblasts and their culture medium by remote detection; (b) remote detection Raman spectrum of human skin fibroblasts with GNPs by removing the optical fiber background and the Raman peaks of the culture medium
Fig. 5. Raman spectra of human skin fibroblasts (a) with and (b) without GNPs by direct detection
Table 1. Characteristic peaks of SERS of human skin fibroblasts
View tableTable 1. Characteristic peaks of SERS of human skin fibroblasts
Raman peak/cm-1 Attribution Direct Direct (GNPs) Remote 584 Phosphatidylinositol 622 C—C twisting mode of phenylalanine (proteins) 636 636 ν (C—S)gauche (aminoacid methionine)660 C—S stretching mode of cystine (collagen type I) 722 Deoxyribonucleic acid (DNA) 754 742 T[ring breathing mode of DNA/ribonucleic acid (RNA) bases] DNA 794 C'5—O—P—O—C'3 phosphodiester bands in DNA 836 833 Proline, hydroxyproline,tyrosine, ν 2Pstretch of nucleic acids 874 Hydroxyproline, tryptophan 892 Backbone, C—C skeletal 943 Proline (collagen tpye Ⅰ) 998 C—O ribose, C—C 1000 1003 1004 Phenylalanine, C—C skeletal 1032 1029 1033 Phenylalanine of collagen 1066 1070 Triglycerides (fatty acids) 1095 Lipid 1146 1146 Carbohydrates peak for solids 1154 C—O stretching, C—N stretching (protein) 1180 1180 1191 Cytosine, guanine, adenine 1201 1208 Tryptophan 1221 Amide III (arising from coupling of C—N stretching) 1244 Asymmetric phosphate [P (asym.)] stretching 1262 T, A (ring breathing modes of the DNA/RNA) 1282 Differences in collagen content 1322 1320 1314 G (DNA/RNA) 1340 1343 CH3, CH2 wagging (collagen assignment) glucose 1370 Guanine, TRP (protein), porphyrins, lipids 1382 δ CH3 symmetric (lipid assignment)05 νs COO-(IgG) 1430 G, A (DNA, RNA) 1451 1456 1451 Deoxyribose 1500 C=C stretching in benzenoid ring 1516 Cytosine 1559 1562 Tryptophan 1582 1580 C—C stretching 1591 1591 Tryptophan 1601 δ (C=C), phenylalanine (protein assignment)1653 Lipid (C=C stretch) 1687 Amide I (disordered structure; non hydrogen bonded)
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Zhao Chunqin, Chen Na, Chen Zhenyi, Zhang Heng, Liu Shupeng, Wang Tingyun. Remote Raman Spectra Detection of Human Skin Fibroblasts[J]. Chinese Journal of Lasers, 2018, 45(5): 507001
Paper Information
Category: biomedical photonics and laser medicine
Received: Sep. 15, 2017
Accepted: --
Published Online: May. 2, 2018
The Author Email: Na Chen (na.chen@shu.edu.cn)
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